The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition
Open accessAbstract
The Toll-like receptor (TLR) family consists of phylogenetically conserved transmembrane proteins, which function as mediators of innate immunity for recognition of pathogen-derived ligands and subsequent cell activation via the Toll/IL-1R signal pathway. Here, we show that human TLR9 (hTLR9) expression in human immune cells correlates with responsiveness to bacterial deoxycytidylate-phosphate-deoxyguanylate (CpG)-DNA. Notably “gain of function” to immunostimulatory CpG-DNA is achieved by expressing TLR9 in human nonresponder cells. Transfection of either human or murine TLR9 conferred responsiveness in a CD14- and MD2-independent manner, yet required species-specific CpG-DNA motifs for initiation of the Toll/IL-1R signal pathway via MyD88. The optimal CpG motif for hTLR9 was GTCGTT, whereas the optimal murine sequence was GACGTT. Overall, these data suggest that hTLR9 conveys CpG-DNA responsiveness to human cells by directly engaging immunostimulating CpG-DNA.
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1 retracted
RETRACTED: DNA-PKcs Is Required for Activation of Innate Immunity by Immunostimulatory DNA
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RetractedDefective LPS Signaling in C3H/HeJ and C57BL/10ScCr Mice: Mutations in Tlr4 Gene
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A Toll-like receptor recognizes bacterial DNA
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CpG motifs in bacterial DNA trigger direct B-cell activation
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Self-citation
Cited by
Takeda, K., Kaisho, T., & Akira, S. (2003) · Annual Review of Immunology · doi:10.1146/annurev.immunol.21.120601.141126 · Cited by 6,143
Self-citationToll-like receptors: critical proteins linking innate and acquired immunity
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Self-citationToll-like receptors and innate immunity
Medzhitov, R. (2001) · Nature Reviews Immunology · doi:10.1038/35100529 · Cited by 4,308
Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8
Heil, F., Hemmi, H., Hochrein, H., Ampenberger, F., Kirschning, C., Akira, S., Lipford, G., Wagner, H., & Bauer, S. (2004) · Science · doi:10.1126/science.1093620 · Cited by 3,947
Self-citationThe immunopathogenesis of sepsis
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Metadata
- Authors
- Bauer, S., Kirschning, C. J., Häcker, H., Redecke, V., Hausmann, S., Akira, S., Wagner, H., & Lipford, G. B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-07-24
- Volume
- 98
- Issue
- 16
- Pages
- 9237-9242
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2069981713
- Cited by
- 1,533
- References
- 44
- FWCI
- 31.71
- Type
- journal article
- Language
- en
- Open access
- green
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- Bauer, S., Kirschning, C. J., Häcker, H., Redecke, V., Hausmann, S., Akira, S., Wagner, H., & Lipford, G. B. (2001). Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.161293498
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