Cited by 400Reproductive tract infections researchPreterm Birth and ChorioamnionitisGut microbiota and health

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Replication and refinement of a vaginal microbial signature of preterm birth in two racially distinct cohorts of US women

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Callahan, Benjamin J., DiGiulio, Daniel B., Goltsman, Daniela S. Aliaga, Sun, Christine L., Costello, Elizabeth K., Jeganathan, Pratheepa, Biggio, Joseph R., Wong, Ronald J., Druzin, Maurice L., Shaw, Gary M., Stevenson, David K., Holmes, Susan P., Relman, David A.·2017·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1705899114PMC PMC5604014

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Abstract

Significance: Premature birth (PTB) is a major global public health burden. Previous studies have suggested an association between altered vaginal microbiota composition and PTB, although findings across studies have been inconsistent. To address these inconsistencies, improve upon our previous signature, and better understand the vaginal microbiota’s role in PTB, we conducted a case-control study in two cohorts of pregnant women: one predominantly Caucasian at low risk of PTB, the second predominantly African American at high risk. With the results, we were able to replicate our signature in the first cohort and refine our signature of PTB for both cohorts. Our findings elucidate the ecology of the vaginal microbiota and advance our ability to predict and understand the causes of PTB.

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Additional file 3 of Meta-analysis reveals the vaginal microbiome is a better predictor of earlier than later preterm birth
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Additional file 4 of Meta-analysis reveals the vaginal microbiome is a better predictor of earlier than later preterm birth
Huang, C. D., Gin, C., Fettweis, J. M., Foxman, B., Gelaye, B., MacIntyre, D. A., Subramaniam, A., Fraser, W., Tabatabaei, N., & Callahan, B. J. (2023) · Figshare · doi:10.6084/m9.figshare.24189161
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Additional file 6 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens
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Additional file 3 of Phylogeny-guided microbiome OTU-specific association test (POST)
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Additional file 3 of Vaginal flora during pregnancy and subsequent risk of preterm birth or prelabor rupture of membranes: a nested case–control study from China
Liu, X., Si, S., Huang, L., Zhang, M., Chen, W., Wang, L., & Yu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26581757
Additional file 2 of Vaginal flora during pregnancy and subsequent risk of preterm birth or prelabor rupture of membranes: a nested case–control study from China
Liu, X., Si, S., Huang, L., Zhang, M., Chen, W., Wang, L., & Yu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26581754
Additional file 1 of Perinatal colonization with extended-spectrum beta-lactamase-producing and carbapenem-resistant Gram-negative bacteria: a hospital-based cohort study
Styczynski, A., Amin, M. B., Hoque, K. I., Parveen, S., Md Pervez, A. F., Zeba, D., Akhter, A., Pitchik, H. O., Islam, M. A., Hossain, M. I., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26673666
Additional file 2 of The association between obesity and weight loss after bariatric surgery on the vaginal microbiota
Raglan, O., MacIntyre, D. A., Mitra, A., Lee, Y. S., Smith, A., Assi, N., Nautiyal, J., Purkayastha, S., Gunter, M. J., Gabra, H., et al. (2021) · Figshare · doi:10.6084/m9.figshare.14700449
Additional file 2 of Composition of the vaginal microbiota during pregnancy in women living in sub-Saharan Africa: a PRISMA-compliant review
Juliana, N. C. A., Peters, R. P. H., Al‐Nasiry, S., Budding, A. E., Morré, S. A., & Ambrosino, E. (2021) · Figshare · doi:10.6084/m9.figshare.16570208
Additional file 7 of Phylogeny-guided microbiome OTU-specific association test (POST)
Huang, C. D., Callahan, B. J., Wu, M. C., Holloway, S. T., Brochu, H. N., Lu, W., Peng, X., & Tzeng, J. (2022) · Figshare · doi:10.6084/m9.figshare.20012397
Self-citation
Additional file 1 of Meta-analysis reveals the vaginal microbiome is a better predictor of earlier than later preterm birth
Huang, C. D., Gin, C., Fettweis, J. M., Foxman, B., Gelaye, B., MacIntyre, D. A., Subramaniam, A., Fraser, W., Tabatabaei, N., & Callahan, B. J. (2023) · Figshare · doi:10.6084/m9.figshare.24189152
Self-citation
Additional file 6 of Phylogeny-guided microbiome OTU-specific association test (POST)
Huang, C. D., Callahan, B. J., Wu, M. C., Holloway, S. T., Brochu, H. N., Lu, W., Peng, X., & Tzeng, J. (2022) · Figshare · doi:10.6084/m9.figshare.20012394
Self-citation
Performance Characteristics of Current Biomarkers for the Prediction of Spontaneous Preterm Birth
Kirschen, G. W., & Gerson, K. D. (2025) · Clinical Chemistry · doi:10.1093/clinchem/hvaf141
Vaginal Microbiome and Preterm Birth in Pregnant Indian WomenPreprint
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Cunningham, M. E., Williams, M. T., Spaine, K. M., Marcinkowska, A., Matveyev, A. V., Hur, B., Natterer, H., Chatterjee, R. B., Oldfield, C. J., Edupuganti, L., et al. (2026) · Research Square · doi:10.21203/rs.3.rs-9268667/v1
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Roth‐Schulze, A. J., Ngui, K., Martin, G., Ashwood, P., Thomson, R. L., Zozaya‐Valdés, E., Gao, Y., Wentworth, J. M., Craig, M. E., Huynh, A., et al. (2026) · Diabetologia · doi:10.1007/s00125-026-06791-6
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Authors: Callahan, B. J., DiGiulio, D. B., Goltsman, D. S. A., Sun, C. L., Costello, E. K., Jeganathan, P., Biggio, J. R., Wong, R. J., Druzin, M. L., Shaw, G. M., Stevenson, D. K., Holmes, S. P., & Relman, D. A.
Affiliations: North Carolina State University (US)
VA Palo Alto Health Care System (US)
Stanford Medicine (US)
Stanford University (US)
University of Alabama at Birmingham (US)
ORCID: Callahan, Benjamin J.
Goltsman, Daniela S. Aliaga
Sun, Christine L.
Costello, Elizabeth K.
Jeganathan, Pratheepa
Biggio, Joseph R.
Wong, Ronald J.
Druzin, Maurice L.
Shaw, Gary M.
Stevenson, David K.
Holmes, Susan P.
Relman, David A.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2017-08-28
Volume: 114
Issue: 37
Pages: 9966-9971
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1705899114
PMC: PMC5604014
OpenAlex: W2749873900
Cited by: 391
References: 48
Citations by year: 2026: 24 · 2025: 28 · 2024: 49 · 2023: 54 · 2022: 62 · 2021: 55 · 2020: 49 · 2019: 37 · 2018: 29 · 2017: 3
FWCI: 26.16
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.pnas.org/site/misc/userlicense.xhtml (vor)
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: March of Dimes Foundation (Prematurity Research Center at Stanford)
HHS | NIH | National Center for Advancing Translational Sciences (UL1 TR001085)
Topics: Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
Health Sciences › Medicine › Obstetrics and Gynecology › Preterm Birth and Chorioamnionitis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords: preterm birth, vaginal microbiota, microbial signatures, bacterial vaginosis, African American women, white women, racial differences, pregnancy, microbial ecology, preterm birth prediction
MeSH terms: Adult, Black or African American, DNA Replication, Female, Gardnerella vaginalis, Humans, Lactobacillus, Pregnancy, RNA, Ribosomal, 16S, United States, Vagina, Cohort Studies, Case-Control Studies, White People, Premature Birth, Microbiota

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Replication and refinement of a vaginal microbial signature of preterm birth in two racially distinct cohorts of US women

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