The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
DNA microarray reveals changes in gene expression of shear stressed human umbilical vein endothelial cells
Open accessAbstract
Using DNA microarray screening (GeneFilter 211, Research Genetics, Huntsville, AL) of mRNA from primary human umbilical vein endothelial cells (HUVEC), we identified 52 genes with significantly altered expression under shear stress [25 dynes/cm 2 for 6 or 24 h (1 dyne = 10 μN), compared with matched stationary controls]; including several genes not heretofore recognized to be shear stress responsive. We examined mRNA expression of nine genes by Northern blot analysis, which confirmed the results obtained on DNA microarrays. Thirty-two genes were up-regulated (by more than 2-fold), the most enhanced being cytochromes P450 1A1 and 1B1, zinc finger protein EZF/GKLF, glucocorticoid-induced leucine zipper protein, argininosuccinate synthase, and human prostaglandin transporter. Most dramatically decreased (by more than 2-fold) were connective tissue growth factor, endothelin-1, monocyte chemotactic protein-1, and spermidine/spermine N1-acetyltransferase. The changes observed suggest several potential mechanisms for increased NO production under shear stress in endothelial cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Atherosclerosis--an inflammatory disease
Ross, R. (1999) · New England Journal of Medicine · doi:10.1056/nejm199901143400207 · Cited by 21,725
Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation
Dimmeler, S., Fleming, I., Fisslthaler, B., Hermann, C., Busse, R., & Zeiher, A. M. (1999) · Nature · doi:10.1038/21224 · Cited by 3,591
Flow-mediated endothelial mechanotransduction
Davies, P. F. (1995) · Physiological Reviews · doi:10.1152/physrev.1995.75.3.519 · Cited by 2,802
Cited by
Chiu, J.-J., & Chien, S. (2011) · Physiological Reviews · doi:10.1152/physrev.00047.2009 · Cited by 2,280
Mammalian Krüppel-Like Factors in Health and Diseases
McConnell, B. B., & Yang, V. W. (2010) · Physiological Reviews · doi:10.1152/physrev.00058.2009 · Cited by 1,018
Molecular basis of the effects of shear stress on vascular endothelial cells
Li, Y.-S. J., Haga, J. H., & Chien, S. (2005) · Journal of Biomechanics · doi:10.1016/j.jbiomech.2004.09.030 · Cited by 904
Role of Integrins in Endothelial Mechanosensing of Shear Stress
Shyy, J. Y.-J., & Chien, S. (2002) · Circulation Research · doi:10.1161/01.res.0000038487.19924.18 · Cited by 549
Role of Krüppel-Like Transcription Factors in Endothelial Biology
Atkins, G. B., & Jain, M. K. (2007) · Circulation Research · doi:10.1161/01.res.0000267856.00713.0a · Cited by 419
Metadata
- Authors
- McCormick, S. M., Eskin, S. G., McIntire, L. V., Teng, C. L., Lu, C.-M., Russell, C. G., & Chittur, K. K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-07-31
- Volume
- 98
- Issue
- 16
- Pages
- 8955-8960
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2130933573
- Cited by
- 385
- References
- 3
- FWCI
- 18.05
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: DNA microarray reveals changes in gene expression of shear stressed human umbilical vein endothelial cells. https://app.cochat.ai/papers/10.1073/pnas.171259298
- The paper
- McCormick, S. M., Eskin, S. G., McIntire, L. V., Teng, C. L., Lu, C.-M., Russell, C. G., & Chittur, K. K. (2001). DNA microarray reveals changes in gene expression of shear stressed human umbilical vein endothelial cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.171259298
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
