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Cited by 316Skin Protection and AgingNonmelanoma Skin Cancer StudiesTea Polyphenols and Effects

Topical applications of caffeine or (-)-epigallocatechin gallate (EGCG) inhibit carcinogenesis and selectively increase apoptosis in UVB-induced skin tumors in mice

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Lu, Yao-Ping, Lou, You-Rong, Xie, Jian-Guo, Peng, Qing-Yun, Liao, Jie, Yang, Chung S., Huang, Mou-Tuan, Conney, Allan H.·2002·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.182429899PMC PMC129466
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Abstract

SKH-1 hairless mice were irradiated with ultraviolet B (UVB) twice weekly for 20 weeks. These tumor-free mice, which had a high risk of developing skin tumors during the next several months, were then treated topically with caffeine (6.2 μmol) or (−)-epigallocatechin gallate (EGCG; 6.5 μmol) once a day 5 days a week for 18 weeks in the absence of further treatment with UVB. Topical applications of caffeine to these mice decreased the number of nonmalignant and malignant skin tumors per mouse by 44% and 72%, respectively. Topical applications of EGCG decreased the number of nonmalignant and malignant tumors per mouse by 55% and 66%, respectively. Immunohistochemical analysis showed that topical applications of caffeine or EGCG increased apoptosis as measured by the number of caspase 3-positive cells in nonmalignant skin tumors by 87% or 72%, respectively, and in squamous cell carcinomas by 92% or 56%, respectively, but there was no effect on apoptosis in nontumor areas of the epidermis. Topical applications of caffeine or EGCG had a small inhibitory effect on proliferation in nonmalignant tumors as measured by BrdUrd labeling (16–22%), and there was also a similar, but nonsignificant, inhibitory effect on proliferation in malignant tumors. The results suggest a need for further studies to determine whether topical applications of caffeine or EGCG can inhibit sunlight-induced skin cancer in humans.

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  • Green tea polyphenols protect against photocarcinogenesis.

    “| (epicatechin-3-gallate), | from tea | Photocarcinogenesis | | + | (117-119) |”

    From Photoprotection: a Review of the Current and Future Technologies (2010) · cites this paper as [119]

    The Abstract reports that topical EGCG, a tea constituent, reduced skin tumors in UVB-pretreated mice, and the Discussion describes inhibition of UVB-induced carcinogenesis. This supports the claim for EGCG in mice, but the study does not establish protection by green tea polyphenols as a class or in humans. (LLM, conf=0.82)

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Cited by

Sorted by citations

Authors
Lu, Y.-P., Lou, Y.-R., Xie, J.-G., Peng, Q.-Y., Liao, J., Yang, C. S., Huang, M.-T., & Conney, A. H.
Affiliations
Rutgers, The State University of New Jersey (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2002-08-30
Volume
99
Issue
19
Pages
12455-12460
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.182429899
PMC
PMC129466
OpenAlex
W2066022538
Cited by
316
References
1
Citations by year
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FWCI
22.06
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (CA88961)
  • National Cancer Institute (P01CA088961, CA 88961)
Topics
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Health Sciences › Medicine › Epidemiology › Nonmelanoma Skin Cancer Studies
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Tea Polyphenols and Effects
Keywords
caffeine, epigallocatechin gallate, topical application, skin carcinogenesis, apoptosis, caspase-3, squamous cell carcinoma, cell proliferation, SKH-1 mice, photoprotection
MeSH terms
Administration, Topical, Animals, Bromodeoxyuridine, Caffeine, Catechin, Female, Mice, Hairless, Neoplasms, Radiation-Induced, Skin Neoplasms, Tea, Ultraviolet Rays, Apoptosis, Caspases, Mice, Caspase 3

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