HSATII RNA is induced via a noncanonical ATM-regulated DNA damage response pathway and promotes tumor cell proliferation and movement
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Abstract
Significance: HSATII RNA is associated with cancer progression and immunostimulation, and as we recently reported, it plays an important role in herpesvirus infections. However, our understanding of cellular processes responsible for the expression of HSATII RNA is limited. Our current investigation identified a noncanonical, ATM kinase-independent DNA damage response pathway as a common cellular mechanism regulating HSATII RNA induction in virus-infected cells or cells treated with DNA-damaging agents. Additionally, our study provides a link between expression of HSATII RNA and the cellular growth and migration phenotypes of cancer cells, establishing a paradigm to study the biological consequences of HSATII RNA expression, i.e., treatment of normal diploid and tumor cells with DNA-damaging agents.
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Noncoding hSATII RNA is highly expressed in most cancer cells and tissues.
“Previous reports demonstrated that noncoding hSATII RNA is highly expressed in most cancer cells and tissues (27–32).”
From Chromatin conformational changes at human satellite II contribute to the senescence phenotype in the tumor microenvironment (2023) · cites this paper as [30]
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Human Satellite 1 (HSAT1) analysis provides novel evidence of pericentromeric transcriptionPreprint
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Additional file 1 of Human Satellite 1A analysis provides evidence of pericentromeric transcription
Lopes, M., Louzada, S., Ferreira, D., Veríssimo, G., Eleutério, D., Gama‐Carvalho, M., & Chaves, R. (2023) · Figshare · doi:10.6084/m9.figshare.22603790
Vojvoda Zeljko, T., Ugarković, Đ., & Pezer, Ž. (2021) · Figshare · doi:10.6084/m9.figshare.16829852
Rodríguez-Martín, F. J., Masero-Leon, M., & Gómez-Cabello, D. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.04.08.714730
- Authors
- Nogalski, M. T., & Shenk, T.
- Affiliations
- Princeton University (US)
- Corresponding author
- Nogalski, Maciej T.
- Shenk, Thomas
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2020-11-30
- Volume
- 117
- Issue
- 50
- Pages
- 31891-31901
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC7749351
- OpenAlex
- W3109989542
- Cited by
- 25
- References
- 120
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 9 · 2022: 4 · 2021: 5
- FWCI
- 1.46
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- HHS | National Institutes of Health (AI112951, AI142520)
- American Cancer Society (PF-14-116-01-MPC)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
- Keywords
- ATM kinase, DNA damage response, human cytomegalovirus, viral replication, cell migration, cell proliferation, breast cancer, colon cancer, pericentromeric repeats, etoposide, zeocin
- MeSH terms
- RNA-Seq, Bleomycin, Breast Neoplasms, Cell Movement, Cytomegalovirus Infections, DNA Damage, DNA Repair, DNA, Satellite, Etoposide, Female, Humans, Repetitive Sequences, Nucleic Acid, Transcriptional Activation, Gene Expression Regulation, Neoplastic, Disease Progression, RNA, Untranslated, Cell Line, Tumor, Cell Proliferation, Gene Knockdown Techniques, Genetic Fitness, Ataxia Telangiectasia Mutated Proteins
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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