Localization and roles of Ski8p protein in Sordaria meiosis and delineation of three mechanistically distinct steps of meiotic homolog juxtaposition
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Ski8p is implicated in degradation of non-poly(A) and double-stranded RNA, and in meiotic DNA recombination. We have identified the Sordaria macrospora SKI8 gene. Ski8p is cytoplasmically localized in all vegetative and sexual cycle cells, and is nuclear localized, specifically in early-mid-meiotic prophase, in temporal correlation with Spo11p, the meiotic double-strand break (DSB) transesterase. Localizations of Ski8p and Spo11p are mutually interdependent. ski8 mutants exhibit defects in vegetative growth, entry into the sexual program, and sporulation. Diverse meiotic defects, also seen in spo11 mutants, are diagnostic of DSB absence, and they are restored by exogenous DSBs. These results suggest that Ski8p promotes meiotic DSB formation by acting directly within meiotic prophase chromosomes. Mutant phenotypes also divide meiotic homolog juxtaposition into three successive, mechanistically distinct steps; recognition, presynaptic alignment, and synapsis, which are distinguished by their differential dependence on DSBs.
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The model of Spo11-Ski8 resembles a previous model based on the Ski3-Ski8 complex, with Ski8 contacting a sequence in Ski3 similar to QREIF380 in Spo11, but suggests a more extensive interaction surface involving an insertion in Ski8 present in Saccharomyces species but not in Schizosaccharomyces pombe and Sordaria macrospora.
“Our model resembles a previous model based on the Ski3–Ski8 complex, with Ski8 contacting a sequence in Ski3 that is similar to the sequence QREIF380 in Spo11 (27,28) (fig. S17A), but suggests a more extensive interaction surface than previously appreciated, involving an insertion in Ski8 that is present in Saccharomyces species but not in Schizosaccharomyces pombe and Sordaria macrospora, where”
From Computed structures of core eukaryotic protein complexes (2021) · cites this paper as [30]
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- Authors
- Tessé, S., Storlazzi, A., Kleckner, N., Gargano, S., & Zickler, D.
- Affiliations
- Université Paris-Sud (FR)
- ORCID
- Kleckner, Nancy
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2003-10-16
- Volume
- 100
- Issue
- 22
- Pages
- 12865-12870
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC240710
- OpenAlex
- W2035696868
- Cited by
- 132
- References
- 37
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 5 · 2022: 5 · 2021: 8 · 2020: 2 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 5 · 2015: 4 · 2014: 9 · 2013: 5 · 2012: 4
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- Funders
- National Institute of General Medical Sciences (R37GM025326, GM 25326, R01GM025326)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- Sordaria macrospora, meiotic double-strand breaks, Spo11, meiotic prophase, meiotic recombination, synapsis, sporulation, nuclear localization
- MeSH terms
- Chromosome Mapping, Cloning, Molecular, Fungal Proteins, Meiosis, Models, Biological, Nuclear Proteins, Recombinant Proteins, Spores, Fungal, Chromosomes, Fungal, Mutagenesis, Sordariales
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