The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Catalysis at a dinuclear [CuSMo(O)OH] cluster in a CO dehydrogenase resolved at 1.1-Å resolution
Open accessAbstract
The CO dehydrogenase of the eubacterium Oligotropha carboxidovorans is a 277-kDa Mo- and Cu-containing iron-sulfur flavoprotein. Here, the enzyme's active site in the oxidized or reduced state, after inactivation with potassium cyanide or with n-butylisocyanide bound to the active site, has been reinvestigated by multiple wavelength anomalous dispersion measurements at atomic resolution, electron spin resonance spectroscopy, and chemical analyses. We present evidence for a dinuclear heterometal [CuSMo( O)OH] cluster in the active site of the oxidized or reduced enzyme, which is prone to cyanolysis. The cluster is coordinated through interactions of the Mo with the dithiolate pyran ring of molybdopterin cytosine dinucleotide and of the Cu with the Sγ of Cys-388, which is part of the active-site loop VAYRC 388 SFR. The previously reported active-site structure [Dobbek, H., Gremer, L., Meyer, O. & Huber, R. (1999) Proc. Natl. Acad. Sci. USA 96, 8884–8889] of an Mo with three oxygen ligands and an SeH-group bound to the Sγ atom of Cys-388 could not be confirmed. The structure of CO dehydrogenase with the inhibitor n-butylisocyanide bound has led to a model for the catalytic mechanism of CO oxidation which involves a thiocarbonate-like intermediate state. The dinuclear [CuSMo( O)OH] cluster of CO dehydrogenase establishes a previously uncharacterized class of dinuclear molybdoenzymes containing the pterin cofactor.
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A Cobalt–Dithiolene Complex for the Photocatalytic and Electrocatalytic Reduction of Protons
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Metadata
- Authors
- Dobbek, H., Gremer, L., Kiefersauer, R., Huber, R., & Meyer, O.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-12-05
- Volume
- 99
- Issue
- 25
- Pages
- 15971-15976
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1973458202
- Cited by
- 386
- References
- 29
- FWCI
- 14.88
- Type
- journal article
- Language
- en
- Open access
- green
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- Dobbek, H., Gremer, L., Kiefersauer, R., Huber, R., & Meyer, O. (2002). Catalysis at a dinuclear [CuSMo(O)OH] cluster in a CO dehydrogenase resolved at 1.1-Å resolution. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.212640899
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