The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Principles of cell-free genetic circuit assembly
Open accessAbstract
Cell-free genetic circuit elements were constructed in a transcription–translation extract. We engineered transcriptional activation and repression cascades, in which the protein product of each stage is the input required to drive or block the following stage. Although we can find regions of linear response for single stages, cascading to subsequent stages requires working in nonlinear regimes. Substantial time delays and dramatic decreases in output production are incurred with each additional stage because of a bottleneck at the translation machinery. Faster turnover of RNA message can relieve competition between genes and stabilize output against variations in input and parameters.
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Cites
Network motifs: simple building blocks of complex networks.
Milo, R., Shen-Orr, S., Itzkovitz, S., Kashtan, N., Chklovskii, D., & Alon, U. (2002) · Science · doi:10.1126/science.298.5594.824 · Cited by 7,368
Transcriptional Regulatory Networks in Saccharomyces cerevisiae
Lee, T. I., Rinaldi, N. J., Robert, F., Odom, D. T., Bar-Joseph, Z., Gerber, G. K., Hannett, N. M., Harbison, C. T., Thompson, C. M., Simon, I., et al. (2002) · Science · doi:10.1126/science.1075090 · Cited by 2,956
Cited by
A vesicle bioreactor as a step toward an artificial cell assembly
Noireaux, V., & Libchaber, A. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408236101 · Cited by 1,152
Self-citationCell-free gene expression: an expanded repertoire of applications
Silverman, A. D., Karim, A. S., & Jewett, M. C. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-019-0186-3 · Cited by 593
Materials learning from life: concepts for active, adaptive and autonomous molecular systems
Merindol, R., & Walther, A. (2017) · Chemical Society Reviews · doi:10.1039/c6cs00738d · Cited by 533
Cell-free synthetic biology: Thinking outside the cell
Hodgman, C. E., & Jewett, M. C. (2012) · Metabolic Engineering · doi:10.1016/j.ymben.2011.09.002 · Cited by 425
An integrated cell‐free metabolic platform for protein production and synthetic biology
Jewett, M. C., Calhoun, K. A., Voloshin, A., Wuu, J. J., & Swartz, J. R. (2008) · Molecular Systems Biology · doi:10.1038/msb.2008.57 · Cited by 384
Metadata
- Authors
- Noireaux, V., Bar-Ziv, R., & Libchaber, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2003-10-14
- Volume
- 100
- Issue
- 22
- Pages
- 12672-12677
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2087993212
- Cited by
- 270
- References
- 2
- FWCI
- 1.39
- Type
- journal article
- Language
- en
- Open access
- green
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