CoChat
Sign Up
Cited by 391Hemoglobinopathies and Related DisordersNitric Oxide and Endothelin EffectsRedox biology and oxidative stress

The DOI matches its Crossref record; 69 of the 69 references it cites were found in a registry or an index.

Oxygen radical inhibition of nitric oxide-dependent vascular function in sickle cell disease

Open access
Aslan, Mutay, Ryan, Thomas M., Adler, Brian, Townes, Tim M., Parks, Dale A., Thompson, J. Anthony, Tousson, Albert, Gladwin, Mark T., Patel, Rakesh P., Tarpey, Margaret M., Batinic-Haberle, Ines, White, C. Roger, Freeman, Bruce A.·2001·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.221292098PMC PMC65009
Open this paper in CoChat — free account

Abstract

Plasma xanthine oxidase (XO) activity was defined as a source of enhanced vascular superoxide (O \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{2}^{{\cdot}-}}}\end{equation*}\end{document} ) and hydrogen peroxide (H 2 O 2 ) production in both sickle cell disease (SCD) patients and knockout-transgenic SCD mice. There was a significant increase in the plasma XO activity of SCD patients that was similarly reflected in the SCD mouse model. Western blot and enzymatic analysis of liver tissue from SCD mice revealed decreased XO content. Hematoxylin and eosin staining of liver tissue of knockout-transgenic SCD mice indicated extensive hepatocellular injury that was accompanied by increased plasma content of the liver enzyme alanine aminotransferase. Immunocytochemical and enzymatic analysis of XO in thoracic aorta and liver tissue of SCD mice showed increased vessel wall and decreased liver XO, with XO concentrated on and in vascular luminal cells. Steady-state rates of vascular O \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{2}^{{\cdot}-}}}\end{equation*}\end{document} production, as indicated by coelenterazine chemiluminescence, were significantly increased, and nitric oxide ( ⋅ NO)-dependent vasorelaxation of aortic ring segments was severely impaired in SCD mice, implying oxidative inactivation of ⋅ NO. Pretreatment of aortic vessels with the superoxide dismutase mimetic manganese 5,10,15,20-tetrakis( N -ethylpyridinium-2-yl)porphyrin markedly decreased O \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{2}^{{\cdot}-}}}\end{equation*}\end{document} levels and significantly restored acetylcholine-dependent relaxation, whereas catalase had no effect. These data reveal that episodes of intrahepatic hypoxia-reoxygenation associated with SCD can induce the release of XO into the circulation from the liver. This circulating XO can then bind avidly to vessel luminal cells and impair vascular function by creating an oxidative milieu and catalytically consuming ⋅ NO via O \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{2}^{{\cdot}-}}}\end{equation*}\end{document} -dependent mechanisms.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1073/pnas.221292098

Cites

69 of 69 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Show all 67 references

Research works printed but not linked: 8

  1. 10.1182/blood.V94.5.1555 · DOI
  2. C O Enwonwu Med Sci Res 17, 997–998 (1989).
  3. C R Morris, F A Kuypers, S Larkin, N Sweeters, J Simon, E P Vichinsky, L A Styles Br J Haematol 111, 498–500 (2000).
  4. S Giler, O Sperling, S Brosh, I Urca, A De Vries Isr J Med Sci 11, 1225 (1975).
  5. K Wolko, J Krawczynski Mater Med Pol 6, 95–98 (1974).
  6. D A Parks, D N Granger Am J Physiol 245, G285–G289 (1983).
  7. Y Yokoyama, J S Beckman, T K Beckman, J K Wheat, T G Cash, B A Freeman, D A Parks Am J Physiol 258, G564–G570 (1990).
  8. E A Kowaluk, P Seth, H L Fung J Pharmacol Exp Ther 262, 916–922 (1992).

Cited by

Sorted by citations

Items 201–300 of 391

Related

See all

Metadata

Authors
Aslan, M., Ryan, T. M., Adler, B., Townes, T. M., Parks, D. A., Thompson, J. A., Tousson, A., Gladwin, M. T., Patel, R. P., Tarpey, M. M., Batinic-Haberle, I., White, C. R., & Freeman, B. A.
Affiliations
  • National Institutes of Health (US)
  • Departments of Anesthesiology, Biochemistry and Molecular Genetics, Pathology, Medicine, and Surgery, Center for Free Radical Biology, Imaging Facility and Comprehensive Sickle Cell Disease Center, University of Alabama, Birmingham, AL 35233; Critical Care Medicine Department of the Warren G. Magnuson Clinical Center, and Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; and Department of...
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2001-12-18
Volume
98
Issue
26
Pages
15215-15220
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.221292098
PMC
PMC65009
OpenAlex
W2014093734
Cited by
391
References
67
Citations by year
2026: 4 · 2025: 5 · 2024: 5 · 2023: 12 · 2022: 9 · 2021: 14 · 2020: 14 · 2019: 18 · 2018: 15 · 2017: 14 · 2016: 9 · 2015: 17 · 2014: 17 · 2013: 16 · 2012: 11
FWCI
9.84
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5p60hl058418-05)
  • National Heart, Lung, and Blood Institute (R01HL64937, R01HL064937, P6-HL-58418, R01HL058115, R01-HL-58115)
Topics
  • Health Sciences › Medicine › Genetics (clinical) › Hemoglobinopathies and Related Disorders
  • Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
sickle cell disease, xanthine oxidase, superoxide, nitric oxide, hydrogen peroxide, hepatocyte injury, aorta, superoxide dismutase mimetic, manganese porphyrin
MeSH terms
Alanine Transaminase, Anemia, Sickle Cell, Animals, Endothelium, Vascular, Erythrocytes, Humans, Muscle Relaxation, Nitric Oxide, Superoxides, Xanthine Oxidase, Mice, Knockout, Mice, In Vitro Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review