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Spartin-mediated lipid transfer facilitates lipid droplet turnover
Open accessPublished version of a preprint
Abstract
Lipid droplets (LDs) are organelles critical for energy storage and membrane lipid homeostasis, whose number and size are carefully regulated in response to cellular conditions. The molecular mechanisms underlying lipid droplet biogenesis and degradation, however, are not well understood. The Troyer syndrome protein spartin (SPG20) supports LD delivery to autophagosomes for turnover via lipophagy. Here, we characterize spartin as a lipid transfer protein whose transfer ability is required for LD degradation. Spartin copurifies with phospholipids and neutral lipids from cells and transfers phospholipids in vitro via its senescence domain. A senescence domain truncation that impairs lipid transfer in vitro also impairs LD turnover in cells while not affecting spartin association with either LDs or autophagosomes, supporting that spartin’s lipid transfer ability is physiologically relevant. Our data indicate a role for spartin-mediated lipid transfer in LD turnover.
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Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Accurate prediction of protein structures and interactions using a 3-track network
Baek, M., DiMaio, F., Anishchenko, I., Dauparas, J., Ovchinnikov, S., Lee, G. R., Wang, J., Cong, Q., Kinch, L. N., Schaeffer, R. D., et al. (2021) · doi:10.1101/2021.06.14.448402 · Cited by 5,549
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Research works printed but not linked: 36
- 10.1038/s41580-018-0085-z · DOI · Paper page
- 10.1016/j.ceb.2023.102178 · DOI
- 10.1083/jcb.202207022 · DOI
- 10.1083/jcb.201807019 · DOI
- 10.1091/mbc.e11-09-0785 · DOI
- 10.1083/jcb.200808041 · DOI
- 10.1038/s41556-023-01178-w · DOI
- 10.1038/ng937 · DOI
- 10.1016/S0092-8674(00)81404-X · DOI
- 10.1021/acsnano.5b06852 · DOI
- 10.1083/jcb.202012058 · DOI
- 10.1038/s41580-018-0071-5 · DOI · Paper page
- 10.1038/nature13269 · DOI
- 10.15252/embj.201797359 · DOI
- 10.1007/s11011-017-0104-3 · DOI
- 10.3390/biom8030045 · DOI
- 10.1038/s41467-023-38725-w · DOI · Paper page
- 10.1126/science.abj8754 · DOI · Paper page
- 10.1042/EBC20220111 · DOI
- 10.1042/BST20220206 · DOI
- 10.1074/jbc.M702824200 · DOI · Paper page
- 10.1242/jcs.259402 · DOI
- 10.1073/pnas.2011442117 · DOI
- 10.1038/nature07976 · DOI · Paper page
- 10.1083/jcb.201811139 · DOI
- B. Ploier, A. K. Menon, A fluorescence-based assay of phospholipid scramblase activity. J. Vis. Exp., 54635 (2016), 10.3791/54635. · DOI
- 10.1006/abio.1994.1112 · DOI
- 10.1021/acs.analchem.2c01446 · DOI
- 10.1007/978-1-0716-1197-5_22 · DOI
- 10.1021/jasms.0c00283 · DOI
- 10.1038/nmeth.4256 · DOI
- 10.1038/s41592-020-0912-y · DOI
- 10.1038/s41592-019-0459-y · DOI
- 10.1093/nar/gkab1038 · DOI · Paper page
- 10.1038/nprot.2013.143 · DOI · Paper page
- 10.1083/jcb.201905162 · DOI
Metadata
- Authors
- Wan, N., Hong, Z., Parson, M. A. H., Korfhage, J. L., Burke, J. E., Melia, T. J., & Reinisch, K. M.
- Affiliations
- Yale University (US)
- University of Victoria (CA)
- University of British Columbia (CA)
- Corresponding author
- Reinisch, Karin M.
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2024-01-08
- Volume
- 121
- Issue
- 3
- Pages
- e2314093121-e2314093121
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC10801920
- OpenAlex
- W4390660086
- Cited by
- 22
- References
- 2
- Citations by year
- 2026: 12 · 2025: 4 · 2024: 6
- FWCI
- 3.52
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (submittedVersion, cc-by)
- Funders
- HHS | NIH | National Institute of General Medical Sciences (R35GM131715, R01135GM135290)
- Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Discovery Grant 2020-04241)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Keywords
- spartin, SPG20, lipid droplets, lipid transfer, lipid droplet turnover, lipophagy, autophagy, phospholipid transfer, lipid droplet formation, lipid homeostasis
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