Cited by 368Immune Response and InflammationChemokine receptors and signalingImmunotherapy and Immune Responses

Flagellin stimulation of intestinal epithelial cells triggers CCL20-mediated migration of dendritic cells

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Sierro, Frédéric, Dubois, Bertrand, Coste, Alix, Kaiserlian, Dominique, Kraehenbuhl, Jean-Pierre, Sirard, Jean-Claude·2001·Proceedings of the National Academy of Sciences

Abstract

Enteropathogenic bacteria elicit mucosal innate and adaptive immune responses. We investigated whether gut epithelial cells played a role in triggering an adaptive immune response by recruiting dendritic cells (DCs). Immature DCs are selectively attracted by the CCL20 chemokine. The expression of the CCL20 gene in human intestinal epithelial cell lines was up-regulated by pathogenic bacteria, including Salmonella species, but not by indigenous bacteria of the intestinal flora. The Salmonella machinery for epithelial cell invasion was not required for CCL20 gene activation. Flagellin but not the lipopolysaccharide was found to be the Salmonella factor responsible for stimulation of epithelial CCL20 production. CCL20 in turn triggered a specific migration of immature DCs. Our data show that crosstalk between bacterial flagellin and epithelial cells is essential for the recruitment of DCs, a mechanism that could be instrumental to initiate adaptive immune responses in the gut.

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Crossref · 10.1073/pnas.241308598

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Authors
Sierro, F., Dubois, B., Coste, A., Kaiserlian, D., Kraehenbuhl, J.-P., & Sirard, J.-C.
Affiliations
  • Inserm (FR)
  • Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, 1066 Epalinges, Switzerland; and Institut National de la Santé et de la Recherche Médicale, U404, 69365 Lyon, France
Venue
Proceedings of the National Academy of Sciences
Published
2001-11-20
Volume
98
Issue
24
Pages
13722-13727
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2061856892
Cited by
368
References
42
Citations by year
2026: 10 · 2025: 2 · 2024: 6 · 2023: 8 · 2022: 3 · 2021: 7 · 2020: 7 · 2019: 11 · 2018: 7 · 2017: 14 · 2016: 12 · 2015: 16 · 2014: 20 · 2013: 14 · 2012: 7
FWCI
6.79
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • IRIS (submittedVersion)
  • SERVAL (Université de Lausanne) (submittedVersion)
Funders
  • National Science Foundation
  • Yale University
  • European Commission
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (56936)
  • Institut National de la Recherche Agronomique
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Keywords
flagellin, intestinal epithelial cells, CCL20, dendritic cell migration, Salmonella, enteropathogenic bacteria, chemokine expression, mucosal immunity, lipopolysaccharide, innate immunity
MeSH terms
Bacteria, Cell Line, Chemotaxis, Culture Media, Dendritic Cells, Epithelial Cells, Flagellin, Heating, Humans, Intestinal Mucosa, Salmonella typhimurium, Gene Expression, Caco-2 Cells, Macrophage Inflammatory Proteins, Receptors, Chemokine, Chemokines, CC, Receptors, CCR6, Chemokine CCL20

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