Flagellin stimulation of intestinal epithelial cells triggers CCL20-mediated migration of dendritic cells
Open accessAbstract
Enteropathogenic bacteria elicit mucosal innate and adaptive immune responses. We investigated whether gut epithelial cells played a role in triggering an adaptive immune response by recruiting dendritic cells (DCs). Immature DCs are selectively attracted by the CCL20 chemokine. The expression of the CCL20 gene in human intestinal epithelial cell lines was up-regulated by pathogenic bacteria, including Salmonella species, but not by indigenous bacteria of the intestinal flora. The Salmonella machinery for epithelial cell invasion was not required for CCL20 gene activation. Flagellin but not the lipopolysaccharide was found to be the Salmonella factor responsible for stimulation of epithelial CCL20 production. CCL20 in turn triggered a specific migration of immature DCs. Our data show that crosstalk between bacterial flagellin and epithelial cells is essential for the recruitment of DCs, a mechanism that could be instrumental to initiate adaptive immune responses in the gut.
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Literature associates CCL20 and IL8 with a pro-inflammatory response when Caco-2 cells are incubated with entero-invasive bacteria such as Salmonella and Shigella.
“In literature, CCL20 and IL8 were associated with a pro-inflammatory response upon incubation of Caco-2 cells with entero-invasive bacteria, such as Salmonella and Shigella [37].”
From Characterization of Vibrio cholerae neuraminidase as an immunomodulator for novel formulation of oral allergy immunotherapy (2018) · cites this paper as [37]
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- Authors
- Sierro, F., Dubois, B., Coste, A., Kaiserlian, D., Kraehenbuhl, J.-P., & Sirard, J.-C.
- Affiliations
- Inserm (FR)
- Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, 1066 Epalinges, Switzerland; and Institut National de la Santé et de la Recherche Médicale, U404, 69365 Lyon, France
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2001-11-20
- Volume
- 98
- Issue
- 24
- Pages
- 13722-13727
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC61108
- OpenAlex
- W2061856892
- Cited by
- 368
- References
- 42
- Citations by year
- 2026: 10 · 2025: 2 · 2024: 6 · 2023: 8 · 2022: 3 · 2021: 7 · 2020: 7 · 2019: 11 · 2018: 7 · 2017: 14 · 2016: 12 · 2015: 16 · 2014: 20 · 2013: 14 · 2012: 7
- FWCI
- 6.79
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Funders
- National Science Foundation
- Yale University
- European Commission
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (56936)
- Institut National de la Recherche Agronomique
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- flagellin, intestinal epithelial cells, CCL20, dendritic cell migration, Salmonella, enteropathogenic bacteria, chemokine expression, mucosal immunity, lipopolysaccharide, innate immunity
- MeSH terms
- Bacteria, Cell Line, Chemotaxis, Culture Media, Dendritic Cells, Epithelial Cells, Flagellin, Heating, Humans, Intestinal Mucosa, Salmonella typhimurium, Gene Expression, Caco-2 Cells, Macrophage Inflammatory Proteins, Receptors, Chemokine, Chemokines, CC, Receptors, CCR6, Chemokine CCL20
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