The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
The membrane protein FeoB contains an intramolecular G protein essential for Fe(II) uptake in bacteria
Open accessAbstract
G proteins are critical for the regulation of membrane protein function and signal transduction. Nevertheless, coupling between G proteins and membrane proteins with multiple membrane-spanning domains has so far been observed only in higher organisms. Here we show that the polytopic membrane protein FeoB, which is essential for Fe(II) uptake in bacteria, contains a guanine-nucleotide-specific nucleotide binding site. We identify the G4-motif, NXXD, responsible for guanine nucleotide specificity, and show that GTP hydrolysis occurs very slowly. In contrast to typical G proteins, the association and dissociation of GDP were found to be faster than for GTP, suggesting that in the absence of additional factors, FeoB's G protein domain may exist mostly in the GTP-bound form. Furthermore, the binding of GTP is required for efficient Fe(II) uptake through the FeoB-dependent system. Notably, even in bacteria, this covalent linkage between a G protein and a polytopic membrane protein appears, to our knowledge, to be unique. These findings raise the intriguing question whether FeoB represents a primordial archetype of G protein-regulated membrane proteins.
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Cites
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Cited by
Andrews, S. C., Robinson, A. K., & Rodríguez-Quiñones, F. (2003) · FEMS Microbiology Reviews · doi:10.1016/s0168-6445(03)00055-x · Cited by 2,702
Bacterial ferrous iron transport: the Feo system
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Iron acquisition strategies of bacterial pathogens
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Bacterial iron detoxification at the molecular level
Bradley, J. M., Svistunenko, D. A., Wilson, M. T., Hemmings, A. M., Moore, G. R., & Le Brun, N. E. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.rev120.007746 · Cited by 140
A new ferrous iron‐uptake transporter, EfeU (YcdN), from Escherichia coli
Große, C., Scherer, J., Koch, D., Otto, M., Taudte, N., & Grass, G. (2006) · Molecular Microbiology · doi:10.1111/j.1365-2958.2006.05326.x · Cited by 137
Metadata
- Authors
- Marlovits, T. C., Haase, W., Herrmann, C., Aller, S. G., & Unger, V. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-11-22
- Volume
- 99
- Issue
- 25
- Pages
- 16243-16248
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2029260500
- Cited by
- 171
- References
- 6
- FWCI
- 1.67
- Type
- journal article
- Language
- en
- Open access
- green
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- Marlovits, T. C., Haase, W., Herrmann, C., Aller, S. G., & Unger, V. M. (2002). The membrane protein FeoB contains an intramolecular G protein essential for Fe(II) uptake in bacteria. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.242338299
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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