Cited by 683Fungal and yeast genetics researchIron Metabolism and DisordersPlant Micronutrient Interactions and Effects

A permease-oxidase complex involved in high-affinity iron uptake in yeast.

Stearman, Robert, Yuan, Daniel S., Yamaguchi-Iwai, Yuko, Klausner, Richard D., Dancis, Andrew·1996·Science

Abstract

Iron must cross biological membranes to reach essential intracellular enzymes. Two proteins in the plasma membrane of yeast--a multicopper oxidase, encoded by the FET3 gene, and a permease, encoded by the FTR1 gene--were shown to mediate high-affinity iron uptake. FET3 expression was required for FTR1 protein to be transported to the plasma membrane. FTR1 expression was required for apo-FET3 protein to be loaded with copper and thus acquire oxidase activity. FTR1 protein also played a direct role in iron transport. Mutations in a conserved sequence motif of FTR1 specifically blocked iron transport.

Integrity signals

Registry

Crossref · 10.1126/science.271.5255.1552

Cites

45 of 45 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • In yeast, the ferrous iron permease FTR1 requires the multicopper ferroxidase FET3 for correct localization on the cell surface.

    “This situation is somewhat analogous to iron transport in yeast, where the ferrous iron permease FTR1 (functionally analogous but structurally unrelated to DMT1) requires the multicopper ferroxidase FET3 for correct localization on the cell surface.85,86”

    From Forging a field: the golden age of iron biology. (2008) · cites this paper as [86]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Stearman, R., Yuan, D. S., Yamaguchi-Iwai, Y., Klausner, R. D., & Dancis, A.
Affiliations
  • National Institutes of Health (US)
  • Cell Biology and Metabolism Branch, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Corresponding author
Dancis, Andrew
Venue
Science
Published
1996-03-15
Volume
271
Issue
5255
Pages
1552-1557
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2005806732
Cited by
683
References
45
Citations by year
2026: 9 · 2025: 16 · 2024: 15 · 2023: 12 · 2022: 26 · 2021: 22 · 2020: 12 · 2019: 10 · 2018: 17 · 2017: 14 · 2016: 13 · 2015: 19 · 2014: 18 · 2013: 21 · 2012: 16
FWCI
16.55
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
Keywords
iron absorption, multicopper oxidase, iron permease, plasma membrane transport, copper loading, yeast, conserved motifs
MeSH terms
Amino Acid Sequence, Binding Sites, Biological Transport, Carrier Proteins, Cell Membrane, Ceruloplasmin, Copper, Endoplasmic Reticulum, Ferric Compounds, Ferritins, Ferrous Compounds, Genes, Fungal, Golgi Apparatus, Iron, Models, Biological, Molecular Sequence Data, Multienzyme Complexes, Mutation, Oxidation-Reduction, Oxidoreductases, Saccharomyces cerevisiae, Transformation, Genetic, Open Reading Frames, Membrane Transport Proteins, Saccharomyces cerevisiae Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review