The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Hybridization of RNA to double-stranded DNA: formation of R-loops.
Open accessAbstract
RNA can hybridize to double-stranded DNA in the presence of 70% formamide by displacing the identical DNA strand. The resulting structure, called an R-loop, is formed in formamide probably because of the greater thermodynamic stability of the RNA-DNA hybrid when it is near the denaturation temperature of duplex DNA. The rate of R-loop formation is maximal at the temperature at which half of the duplex DNA is irreversibly converted to single-stranded DNA (the strand separation temperature of tss) of the duplex DNA and falls precipitously a few degrees above or below that temperature. This maximal rate is similar to the rate of hybridization of RNA to single-stranded DNA under the same conditions. At temperatures above the tss the rate is proportional to the RNA concentration. However, at temperatures below tss the rate of R-loop formation is less dependent upon the RNA concentration. Once formed, the R-loops display considerable stability; the formamide can be removed and the DNA can be cleaved with restriction endonucleases without loss of R-loop structures.
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Cites
Kinetics of renaturation of DNA
Wetmur, J. G., & Davidson, N. (1968) · Journal of Molecular Biology · doi:10.1016/0022-2836(68)90414-2 · Cited by 1,422
Dependence of the melting temperature of DNA on salt concentration
Schildkraut, C., & Lifson, S. (1965) · Biopolymers · doi:10.1002/bip.360030207 · Cited by 691
Viable Molecular Hybrids of Bacteriophage Lambda and Eukaryotic DNA
Thomas, M., Cameron, J. R., & Davis, R. W. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.11.4579 · Cited by 241
Self-citationLee, C. S., Davis, R. W., & Davidson, N. (1970) · Journal of Molecular Biology · doi:10.1016/0022-2836(70)90215-9 · Cited by 222
Self-citationReplication of Mitochondrial DNA. Circular Replicative Intermediates in Mouse L Cells
Robberson, D. L., Kasamatsu, H., & Vinograd, J. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.3.737 · Cited by 211
Cited by
Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ
Benton, W. D., & Davis, R. W. (1977) · Science · doi:10.1126/science.322279 · Cited by 4,595
Self-citationKruger, K., Grabowski, P. J., Zaug, A. J., Sands, J., Gottschling, D. E., & Cech, T. R. (1982) · Cell · doi:10.1016/0092-8674(82)90414-7 · Cited by 2,331
Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes
Cox, K. H., DeLeon, D. V., Angerer, L. M., & Angerer, R. C. (1984) · Developmental Biology · doi:10.1016/0012-1606(84)90162-3 · Cited by 1,611
R-Loops as Cellular Regulators and Genomic Threats
Crossley, M. P., Bocek, M., & Cimprich, K. A. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.01.024 · Cited by 746
Duquette, M. L., Handa, P., Vincent, J. A., Taylor, A. F., & Maizels, N. (2004) · Genes & Development · doi:10.1101/gad.1200804 · Cited by 553
Metadata
- Authors
- Thomas, M., White, R. L., & Davis, R. W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1976-07
- Volume
- 73
- Issue
- 7
- Pages
- 2294-2298
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2031816743
- Cited by
- 488
- References
- 5
- FWCI
- 15.90
- Type
- journal article
- Language
- en
- Open access
- green
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