The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Detection of viral sequences of low reiteration frequency by in situ hybridization.
Open accessAbstract
The sensitivity of in situ hybridization has been increased at least 10-fold by hybridizing in cDNA excess, by increasing the diffusion of the cDNA through the cells, by hybridizing at optimum temperature, and by stabilizing hybrids during autoradiography. Saturation of intracellular RNA with [3H]cDNA has been achieved. The assay is quantitative. In situ hybridization has been used to detect and quantitate visna virus RNA in infected cells. By using [3H]cDNA with specific activity of 2 X 10(8) dpm/micrograms and conditions that reduce background to negligible levels, 10--20 copies of viral RNA per cell can be detected and quantitated after 2 days of autoradiographic exposure.
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Cites
A membrane-filter technique for the detection of complementary DNA
Denhardt, D. T. (1966) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(66)90447-5 · Cited by 3,885
Maniatis, T., Jeffrey, A., & Van deSande, H. (1975) · Biochemistry · doi:10.1021/bi00688a010 · Cited by 1,121
Kinetic complexity of RNA molecules
Birnstiel, M. L., Sells, B. H., & Purdom, I. F. (1972) · Journal of Molecular Biology · doi:10.1016/0022-2836(72)90519-0 · Cited by 386
Slow Persistent Infection Caused by Visna Virus: Role of Host Restriction
Haase, A. T., Stowring, L., Narayan, O., Griffin, D. E., & Price, D. D. (1977) · Science · doi:10.1126/science.188133 · Cited by 165
Self-citationBonner, J. J., & Pardue, M. L. (1976) · Chromosoma · doi:10.1007/bf00293443 · Cited by 159
Imaizumi‐Scherrer, T., Diggelmann, H., & Scherrer, K. (1973) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.70.4.1122 · Cited by 157
The Slow Infection Caused by Visna Virus
Haase, A. T. (1975) · Modern aspects of electrochemistry · doi:10.1007/978-3-642-66289-8_4 · Cited by 141
Self-citationHutton, J. R., & Wetmur, J. G. (1973) · Biochemistry · doi:10.1021/bi00727a032 · Cited by 117
Szabo, P., Elder, R., Steffensen, D. M., & Uhlenbeck, O. C. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90170-x · Cited by 96
Cited by
Melton, D. A., Krieg, P. A., Rebagliati, M. R., Maniatis, T., Zinn, K., & Green, M. R. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.18.7035 · Cited by 6,324
Diedrich, J. F., Minnigan, H., Carp, R. I., Whitaker, J. N., Race, R., Frey, W., & Haase, A. T. (1991) · Journal of Virology · doi:10.1128/jvi.65.9.4759-4768.1991 · Cited by 296
Self-citationLocalization of glucocorticoid receptor mRNA in the male rat brain by in situ hybridization
Aronsson, M., Fuxe, K., Dong, Y., Agnati, L. F., Okret, S., & Gustafsson, J. A. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.23.9331 · Cited by 261
Neuropeptide and small transmitter coexistence: Fundamental studies and relevance to mental illness
Hökfelt, T., Barde, S., Xu, Z.-Q. D., Kuteeva, E., Rüegg, J., Le Maitre, E., Risling, M., Kehr, J., Ihnatko, R., Theodorsson, E., et al. (2018) · Frontiers in Neural Circuits · doi:10.3389/fncir.2018.00106 · Cited by 139
Metadata
- Authors
- Brahic, M., & Haase, A. T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1978-12
- Volume
- 75
- Issue
- 12
- Pages
- 6125-6129
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1980292565
- Cited by
- 451
- References
- 9
- FWCI
- 3.58
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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