The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Acetylcholine and local anesthetic binding to Torpedo nicotinic postsynaptic membranes after removal of nonreceptor peptides.
Open accessAbstract
After alkaline extraction, purified subsynaptic fragments isolated from Torpedo electric tissue exhibit on sodium dodecyl sulfate/polyacrylamide gel electrophoresis predominant peptides of apparent Mr 41,000, 50,000, and 65,000 (i.e., the peptides characteristic of the nicotinic receptor purified and isolated in detergent solutions). The peptide of Mr 43,000 that is also found in the isolated postsynaptic membranes is recovered in the supernatant after alkaline extraction. The alkaline-extracted membranes were functionally intact, as demonstrated by the following criteria. The kinetics of binding of [3H]acetylcholine in the presence and absence of 30 micron carbamoylcholine to occupy acetylcholine binding sites, [14C]-meproadifen [2-(diethylmethylaminoethyl)-2,2-diphenylvalerate iodide ] was bound with a dissociation constant, KD, of 0.3 +/- 0.1 micron to 0.3 +/- 0.1 site per [3H]alpha-toxin site. This binding was displaced by perhydrohistrionicotoxin. The carbamoylcholine-stimulated efflux of 22Na+ from the Torpedo vesicles were preserved after alkaline extraction. It is concluded that not only the acetylcholine binding site, but also the local anesthetic binding site, must be associated with the peptides of the cholinergic receptor itself and not that of Mr 43,000. Those peptides remaining after alkaline extraction are also sufficient for permeability control.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,221
Local anaesthetics transiently block currents through single acetylcholine‐receptor channels.
Neher, E., & Steinbach, J. H. (1978) · The Journal of Physiology · doi:10.1113/jphysiol.1978.sp012267 · Cited by 844
Selective solubilization of proteins from red blood cell membranes by protein perturbants
Steck, T. L., & Yu, J. (1973) · Journal of Supramolecular Structure · doi:10.1002/jss.400010307 · Cited by 607
Heidmann, T., & Changeux, J. P. (1978) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.47.070178.001533 · Cited by 395
SOBEL, A., WEBER, M., & CHANGEUX, J. (1977) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1977.tb11874.x · Cited by 374
Affinity-labeling of purified acetylcholine receptor from Torpedo californica
Weill, C. L., McNamee, M. G., & Karlin, A. (1974) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(74)90254-x · Cited by 244
Voltage jump analysis of procaine action at frog end‐plate
Adams, P. R. (1977) · The Journal of Physiology · doi:10.1113/jphysiol.1977.sp011858 · Cited by 227
Chang, H. W., & Bock, E. (1977) · Biochemistry · doi:10.1021/bi00639a028 · Cited by 184
Kinetics of agonist-mediated transitions in state of the cholinergic receptor.
Weiland, G. A., Georgia, B., Lappi, S. E., Chignell, C. F., & Taylor, P. (1977) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)41016-7 · Cited by 142
Cited by
Cocaine Receptors on Dopamine Transporters Are Related to Self-Administration of Cocaine
Ritz, M. C., Lamb, R. J., Goldberg, S. R., & Kuhar, M. J. (1987) · Science · doi:10.1126/science.2820058 · Cited by 2,180
Acetylcholine Receptor: An Allosteric Protein
Changeux, J.-P., Devillers-Thiéry, A., & Chemouilli, P. (1984) · Science · doi:10.1126/science.6382611 · Cited by 623
Furley, A. J., Morton, S. B., Manalo, D., Karagogeos, D., Dodd, J., & Jessell, T. M. (1990) · Cell · doi:10.1016/0092-8674(90)90223-2 · Cited by 567
Miyazawa, A., Fujiyoshi, Y., Stowell, M., & Unwin, N. (1999) · Journal of Molecular Biology · doi:10.1006/jmbi.1999.2721 · Cited by 490
Dennis, M., Giraudat, J., Kotzyba-Hibert, F., Goeldner, M., Hirth, C., Chang, J. Y., Lazure, C., Chretien, M., & Changeux, J. P. (1988) · Biochemistry · doi:10.1021/bi00407a016 · Cited by 317
Metadata
- Authors
- Neubig, R. R., Krodel, E. K., Boyd, N. D., & Cohen, J. B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1979-02
- Volume
- 76
- Issue
- 2
- Pages
- 690-694
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2031886427
- Cited by
- 349
- References
- 14
- FWCI
- 16.00
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Neubig, R. R., Krodel, E. K., Boyd, N. D., & Cohen, J. B. (1979). Acetylcholine and local anesthetic binding to Torpedo nicotinic postsynaptic membranes after removal of nonreceptor peptides. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.76.2.690
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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