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Fluorescent phallotoxin, a tool for the visualization of cellular actin.
Open accessAbstract
A fluorescent derivative of phalloidin has been synthesized possessing high affinity to filamentous actin. This compound was used for visualization of actin-containing structures in eukaryotic nonmuscle cells. Due to its low molecular weight (1250), fixation for formaldehyde was sufficient to render the membrane permeable for the labeled peptide. Bundles of microfilaments are the predominant pattern in the flat rat kangaroo PtK1 cells, whereas a net of concentric fibers characterizes the more spherical bovine kidney MDBK cells. Specificity of staining was confirmed by competition experiments with unlabeled phalloidin.
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Citations
Cited by
Effects of cytochalasin and phalloidin on actin.
Cooper, J. A. (1987) · The Journal of cell biology · doi:10.1083/jcb.105.4.1473 · Cited by 2,009
Forscher, P., & Smith, S. J. (1988) · The Journal of cell biology · doi:10.1083/jcb.107.4.1505 · Cited by 854
L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein
Kocks, C., Gouin, E., Tabouret, M., Berche, P., Ohayon, H., & Cossart, P. (1992) · Cell · doi:10.1016/0092-8674(92)90188-i · Cited by 817
Spector, I., Shochet, N. R., Blasberger, D., & Kashman, Y. (1989) · Cell Motility · doi:10.1002/cm.970130302 · Cited by 580
IL-6 increases endothelial permeability in vitro
Maruo, N. (1992) · Endocrinology · doi:10.1210/en.131.2.710 · Cited by 386
Metadata
- Authors
- Wulf, E., Deboben, A., Bautz, F. A., Faulstich, H., & Wieland, T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1979-09
- Volume
- 76
- Issue
- 9
- Pages
- 4498-4502
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1968577363
- Cited by
- 786
- References
- 0
- FWCI
- 4.63
- Type
- journal article
- Language
- en
- Open access
- green
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- Wulf, E., Deboben, A., Bautz, F. A., Faulstich, H., & Wieland, T. (1979). Fluorescent phallotoxin, a tool for the visualization of cellular actin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.76.9.4498
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