The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Direct transfer of cloned genes from bacteria to mammalian cells.
Open accessAbstract
Induction of a virus infection by cloned simian virus 40 DNA was chosen as a test system to detect transfer of genes from bacteria to cultured mammalian cells. Escherichia coli cells containing a recombinant plasmid with three tandem inserts of simian virus 40 DNA were able to infect CV-1 monkey cells under various conditions. The gene transfer was resistant to DNase I and therefore seems not to occur via free DNA but most likely via uptake of whole bacteria, followed by release of plasmid DNA and generation of infectious circular simian virus 40 DNA in a recombination-excision process. Spontaneous transfer was found to be infrequent, 4 x 10(9) bacteria yielding one infection per 10(7) monkey cells. The frequency was greatly increased by adding bacteria as a calcium phosphate coprecipitate or by fusion of lysozyme-treated bacteria (protoplasts) with monkey cells in the presence of polyethylene glycol. With the latter technique, 10(4) protoplasts gave rise to one infection per 15 monkey cells. Experiments with other cell lines of human, monkey, and mouse origin, and also with bacteria harboring another recombinant plasmid, indicate that DNA transfer from bacteria to mammalian cells is a general phenomenon.
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Cites
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Cited by
Expression of a β-globin gene is enhanced by remote SV40 DNA sequences
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Self-citationA cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
Okayama, H., & Berg, P. (1983) · Molecular and Cellular Biology · doi:10.1128/mcb.3.2.280 · Cited by 933
Electroporation for the efficient transfection of mammalian cells with DNA
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High efficiency polyoma DNA transfection of chloroquine treated cells
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Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells.
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Metadata
- Authors
- Schaffner, W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1980-04
- Volume
- 77
- Issue
- 4
- Pages
- 2163-2167
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2008416020
- Cited by
- 277
- References
- 20
- FWCI
- 17.21
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
