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Cited by 1,724Genomics and Chromatin DynamicsHemoglobinopathies and Related DisordersChromosomal and Genetic Variations

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Expression of a β-globin gene is enhanced by remote SV40 DNA sequences

Banerji, Julian, Rusconi, Sandro, Schaffner, Walter·1981·Cell
DOI 10.1016/0092-8674(81)90413-x
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Abstract

We have studied the transient expression of a cloned rabbit hemoglobin beta 1 gene after its introduction into HeLa cells. Two and one-half days after transfection using the calcium phosphate technique we extracted RNA from the entire cell population and analyzed it by the S1 nuclease hybridization assay. Transcripts were barely detectable when beta-globin gene-plasmid recombinants were used. However, 200 times more beta-globin gene transcripts were found when the beta-globin gene recombinants also contained SV40 DNA, and 90% of these transcripts (about 1000 per cell) had the same 5' end as authentic rabbit globin mRNA. In the latter case, abundant production of beta-globin protein was readily detected in a fraction of transfected cells by immunofluorescent staining. Enhancement of globin gene expression was dependent on SV40 sequences acting in cis, but independent of the viral origin of DNA replication. The enhancing activity was associated with the 72 bp repeated sequence element located at the beginning of the viral late gene region. Viral DNA fragments containing the transcriptional enhancer element could act in either orientation at many positions, including 1400 bp upstream or 3300 bp downstream from the transcription initiation site of the rabbit beta-globin gene. These studies define a class of DNA elements with a mode of action that has not been heretofore described. The activation of genes by specific enhancer elements seems to be a widespread mechanism that may be used for the regulation of gene expression.

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  • A 72-bp repeat sequence element in SV40 is an enhancing sequence capable of vastly upregulating gene expression from a plasmid upon transfection into mammalian cells.

    “Subsequent landmark experiments by the groups of Walter Schaffner [4] and Pierre Chambon [5] confirmed that a 72-bp repeat sequence element in SV40 [8, 9] was an “enhancing” sequence, capable of vastly upregulating gene expression from a plasmid upon transfection into mammalian cells.”

    From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [4]

  • The SV40 enhancer could activate rabbit or human β-globin genes from varying distances from their promoters, regardless of its orientation.

    “Moreover, this enhancer could activate rabbit or human β-globin genes from varying distances from their promoters, regardless of its orientation [4, 5].”

    From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [4]

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Research works printed but not linked: 38

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Metadata

Authors
Banerji, J., Rusconi, S., & Schaffner, W.
Affiliations
University of Zurich (CH)
Venue
Cell
Publisher
Elsevier BV
Published
1981-12
Volume
27
Issue
2
Pages
299-308
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/0092-8674(81)90413-x
OpenAlex
W2067956561
Cited by
1,724
References
1
Citations by year
2026: 32 · 2025: 74 · 2024: 67 · 2023: 71 · 2022: 50 · 2021: 68 · 2020: 58 · 2019: 60 · 2018: 54 · 2017: 40 · 2016: 49 · 2015: 57 · 2014: 43 · 2013: 24 · 2012: 26
FWCI
54.37
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Health Sciences › Medicine › Genetics (clinical) › Hemoglobinopathies and Related Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
Keywords
beta-globin gene, simian virus 40, enhancers, gene expression regulation, transfection, HeLa cells, calcium phosphate transfection, cis-acting enhancer, mRNA 5' end
MeSH terms
Animals, Antigens, Neoplasm, Antigens, Viral, Antigens, Viral, Tumor, Base Sequence, DNA, Recombinant, DNA, Viral, Fluorescent Antibody Technique, Gene Expression Regulation, Genes, Globins, HeLa Cells, Humans, Kinetics, Rabbits, Ribonucleases, Simian virus 40, Transfection

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