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Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin.
Open accessAbstract
Cultures consisting almost entirely of human melanocytes were obtained from epidermal single-cell suspensions by using phorbol 12-myristate 13-acetate (10 ng/ml) in the culture medium. At this concentration, phorbol ester is toxic to human keratinocytes but not to melanocytes. When the seeding density was optimal (0.8-2 x 10(4)/cm2) and the medium contained both phorbol ester and cholera toxin, melanocytes proliferated extensively. Under these conditions, human melanocytes could be passaged serially in vitro and grown in quantity. This cell culture system can thus be used to answer basic questions related to pigment cell biology and may serve as a control for studies of malignant melanocytes.
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Citations
Cited by
Mitogenic and melanogenic stimulation of normal human melanocytes by melanotropic peptides
Abdel-Malek, Z., Swope, V. B., Suzuki, I., Akcali, C., Harriger, M. D., Boyce, S. T., Urabe, K., & Hearing, V. J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.5.1789 · Cited by 377
Role of Tyrosinase as the Determinant of Pigmentation in Cultured Human Melanocytes
Iozumi, K., Hoganson, G. E., Pennella, R., Everett, M. A., & Fuller, B. B. (1993) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12476630 · Cited by 267
Effects of endothelins on signal transduction and proliferation in human melanocytes.
Yada, Y., Higuchi, K., & Imokawa, G. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55277-7 · Cited by 269
Roméro-Graillet, C., Aberdam, E., Biagoli, N., Massabni, W., Ortonne, J.-P., & Ballotti, R. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.45.28052 · Cited by 178
Targeting the Complexity of In Vitro Skin Models: A Review of Cutting-Edge Developments
Quílez, C., Bebiano, L. B., Jones, E., Maver, U., Meesters, L., Parzymies, P., Petiot, E., Rikken, G., Risueño, I., Zaidi, H., et al. (2024) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2024.04.032 · Cited by 65
Metadata
- Authors
- Eisinger, M., & Marko, O.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1982-03
- Volume
- 79
- Issue
- 6
- Pages
- 2018-2022
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1980384590
- Cited by
- 549
- References
- 0
- FWCI
- 8.08
- Type
- journal article
- Language
- en
- Open access
- green
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- Eisinger, M., & Marko, O. (1982). Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.79.6.2018
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