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Cited by 65Skin and Cellular Biology ResearchAdvancements in Transdermal Drug Delivery3D Printing in Biomedical Research

Targeting the Complexity of In Vitro Skin Models: A Review of Cutting-Edge Developments

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Quílez, Cristina, Bebiano, Luís B., Jones, Eleri, Maver, Uroš, Meesters, Luca, Parzymies, Piotr, Petiot, Emma, Rikken, Gijs +14 more·2024·Journal of Investigative Dermatology
DOI 10.1016/j.jid.2024.04.032
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Abstract

Skin in vitro models offer much promise for research, testing drugs, cosmetics, and medical devices, reducing animal testing and extensive clinical trials. There are several in vitro approaches to mimicking human skin behavior, ranging from simple cell monolayer to complex organotypic and bioengineered 3-dimensional models. Some have been approved for preclinical studies in cosmetics, pharmaceuticals, and chemicals. However, development of physiologically reliable in vitro human skin models remains in its infancy. This review reports on advances in in vitro complex skin models to study skin homeostasis, aging, and skin disease.

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Crossref · 10.1016/j.jid.2024.04.032

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  • Reconstructed skin equivalents and organoid models lack integrated vascular and immune-associated compartments, limiting their capacity to model dynamic inflammatory processes.

    “Although reconstructed skin equivalents and organoid models have advanced our understanding of epidermal differentiation, they lack integrated vascular and immune-associated compartments, limiting their capacity to model dynamic inflammatory processes 38,39 .”

    From Vascularized human skin assembloids model compartmental skin organization and immune responsiveness in vitro (2026) · cites this paper as [39]

    The review says simple 3D skin models lack circulatory flow (New Advances, p. 4) and that cytokine-based models may not capture the dynamics of inflammation in vivo (Inflammatory skin diseases models, p. 11). But it also describes models incorporating immune cells and skin-on-chip models with vascularization (pp. 6, 11). The claim overgeneralizes these limitations to reconstructed skin equivalents and organoids as a whole. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Quílez, C., Bebiano, L. B., Jones, E., Maver, U., Meesters, L., Parzymies, P., Petiot, E., Rikken, G., Risueño, I., Zaidi, H., Zidarič, T., Bekeschus, S., H. van den Bogaard, E., Caley, M., Colley, H., López, N. G., Letsiou, S., Marquette, C., Maver, T., Pereira, R. F., Tobin, D. J., & Velasco, D.
Affiliations
  • Instituto de Investigación Sanitaria Fundación Jiménez Díaz (ES)
  • Universidade do Porto (PT)
  • Queen Mary University of London (GB)
  • University of Maribor (SI)
  • Radboud University Medical Center (NL)
  • Lyon 1 Université (FR)
  • Leibniz Institute for Plasma Science and Technology (DE)
  • University of Sheffield (GB)
  • University of West Attica (GR)
  • University College Dublin (IE)
Corresponding author
Velasco, Diego
ORCID
Venue
Journal of Investigative Dermatology
Publisher
Elsevier BV
Published
2024-12
Volume
144
Issue
12
Pages
2650-2670
ISSN
0022-202X (ISSN-L)
DOI
10.1016/j.jid.2024.04.032
OpenAlex
W4401486232
Cited by
65
References
17
Citations by year
2026: 39 · 2025: 26
FWCI
3.43
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Investigative Dermatology (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Radboud Repository (Radboud University) (submittedVersion, cc-by)
  • HAL (submittedVersion)
Funders
  • European Commission (101118430)
  • Bundesministerium für Bildung und Forschung (03Z22DN11, 03Z22DI1)
  • Javna Agencija za Raziskovalno Dejavnost RS (L7-4494, P3-0036)
  • Universidad Carlos III de Madrid
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
in vitro skin model, organotypic skin model, 3D skin model, skin homeostasis, skin aging, skin diseases, alternatives to animal testing, preclinical testing, cosmetic testing, pharmaceutical testing, bioengineered skin
MeSH terms
Animals, Humans, Models, Biological, Skin, Skin Diseases, Skin Physiological Phenomena, Skin Aging, Tissue Engineering, In Vitro Techniques

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