The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Effects of endothelins on signal transduction and proliferation in human melanocytes.
Open accessAbstract
We demonstrate here that human melanocytes could be regulated by endothelin (ET) derivatives, potent vasoconstrictive peptides synthesized by endothelial cells, to stimulate their proliferation and melanization via a receptor-mediated signal transduction pathway. Receptor-binding assay using [125I]ET indicated that unlabeled ET-1 or ET-2 competitively inhibited each binding of labeled ETs to melanocytes with a concentration for half-maximal inhibition (IC50) of 0.7 or 0.9 nM, respectively. The dissociation constant (Kd) and the number of sites of the specific bindings of ET-1 and those of ET-2 were almost the same (Kd: 1.81 nM, binding sites: 7.0-8.0 x 10(4) per cell). Upon incubation with cultured cells, the mass contents of inositol 1,4,5-trisphosphate and intracellular calcium level were substantially increased by 10 nM ET-1, ET-2, and ET-3, but not by big-ET with maximal response at 80-130-s postincubation. The addition of ET-1 and ET-2 at 1-50 nM concentrations caused human melanocytes to significantly stimulate DNA [( 3H]thymidine incorporation) and melanin synthesis (3H2O release and [14C] thiouracil incorporation). Furthermore, ETs exhibited an additive stimulatory effect on basic fibroblast growth factor-stimulated DNA synthesis. In a long-term serum-free culture system, the strongest stimulation of growth by 10 nM ET-1 or ET-2 was observed in the presence of 10 nM cholera toxin and 0.2% bovine pituitary extract, resulting in a 4.5-fold increase in cell number for 12 culture days. These findings strongly suggest involvement of ET in the mechanism regulating proliferation and melanization of human melanocytes.
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Cites
A novel potent vasoconstrictor peptide produced by vascular endothelial cells
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Basic fibroblast growth factor from human keratinocytes is a natural mitogen for melanocytes.
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The Contributions of UVA and UVB to Connective Tissue Damage in Hairless Mice
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Cited by
Melanin pigmentation in mammalian skin and its hormonal regulation
Slominski, A., Tobin, D. J., Shibahara, S., & Wortsman, J. (2004) · Physiological Reviews · doi:10.1152/physrev.00044.2003 · Cited by 2,015
Endothelins secreted from human keratinocytes are intrinsic mitogens for human melanocytes
Imokawa, G., Yada, Y., & Miyagishi, M. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)35817-4 · Cited by 385
Self-citationAutocrine and paracrine regulation of melanocytes in human skin and in pigmentary disorders
Imokawa, G. (2004) · Pigment Cell Research · doi:10.1111/j.1600-0749.2003.00126.x · Cited by 369
Self-citationChakraborty, A. K., Funasaka, Y., Slominski, A., Ermak, G., Hwang, J., Pawelek, J. M., & Ichihashi, M. (1996) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/0167-4889(96)00063-8 · Cited by 333
Murase, D., Hachiya, A., Takano, K., Hicks, R., Visscher, M. O., Kitahara, T., Hase, T., Takema, Y., & Yoshimori, T. (2013) · Journal of Investigative Dermatology · doi:10.1038/jid.2013.165 · Cited by 214
Metadata
- Authors
- Yada, Y., Higuchi, K., & Imokawa, G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1991-09
- Volume
- 266
- Issue
- 27
- Pages
- 18352-18357
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1519510465
- Cited by
- 269
- References
- 39
- FWCI
- 8.19
- Type
- journal article
- Language
- en
- Open access
- hybrid
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