The DOI matches its Crossref record.
Copper metallothionein of yeast, structure of the gene, and regulation of expression.
Open accessAbstract
Addition of copper to yeast cells leads to the induction of a low molecular weight, cysteine-rich protein that binds copper. This protein, termed copper chelatin or thionein, is related to the metallothionein family of proteins that are induced in response to cadmium and zinc in vertebrate cells. We have determined the structure of the yeast copper-binding protein by DNA sequence analysis of the gene. Although the 6573-dalton yeast protein is substantially divergent from vertebrate metallothioneins, the arrangement of 12 cysteine residues, which is a hallmark of metal-binding proteins, is partially conserved. We analyzed the regulatory DNA sequence of the gene by fusing it with the Escherichia coli galactokinase gene and assaying the levels of enzyme activity in yeast in response to copper. The transcriptional activation has a specific requirement for copper. Zinc, cadmium, and gold were unable to regulate the galactokinase activity. The yeast copper metallothionein regulatory sequences represent a previously unreported class of yeast promoter that is regulated by copper.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Interactions of fungi with toxic metals
GADD, G. M. (1993) · New Phytologist · doi:10.1111/j.1469-8137.1993.tb03796.x · Cited by 1,154
S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP
Normington, K., Kohno, K., Kozutsumi, Y., Gething, M.-J., & Sambrook, J. (1989) · Cell · doi:10.1016/0092-8674(89)90059-7 · Cited by 408
Copper resistance mechanisms in bacteria and fungi
Cervantes, C. (1994) · FEMS Microbiology Reviews · doi:10.1016/0168-6445(94)90002-7 · Cited by 391
ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene.
Thiele, D. J. (1988) · Molecular and Cellular Biology · doi:10.1128/mcb.8.7.2745 · Cited by 310
Metal ion resistance in fungi: Molecular mechanisms and their regulated expression
Mehra, R. K., & Winge, D. R. (1991) · Journal of Cellular Biochemistry · doi:10.1002/jcb.240450109 · Cited by 301
Metadata
- Authors
- Butt, T. R., Sternberg, E. J., Gorman, J. A., Clark, P., Hamer, D., Rosenberg, M., & Crooke, S. T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1984-06
- Volume
- 81
- Issue
- 11
- Pages
- 3332-3336
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2073331932
- Cited by
- 280
- References
- 0
- FWCI
- 13.98
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Copper metallothionein of yeast, structure of the gene, and regulation of expression. https://app.cochat.ai/papers/10.1073/pnas.81.11.3332
- The paper
- Butt, T. R., Sternberg, E. J., Gorman, J. A., Clark, P., Hamer, D., Rosenberg, M., & Crooke, S. T. (1984). Copper metallothionein of yeast, structure of the gene, and regulation of expression. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.11.3332
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
