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Escherichia coli K-12 restricts DNA containing 5-methylcytosine.
Open accessAbstract
We have observed that plasmids containing certain cloned modification methylase genes of type II restriction-modification systems cannot be transformed into many laboratory strains of Escherichia coli K-12. The investigation of this phenomenon, reported here, has revealed (i) DNA containing 5-methylcytosine is biologically restricted by these strains, while DNA containing 6-methyladenine is not; (ii) restriction is due to two genetically distinct systems that differ in their sequence specificities, which we have named mcrA and mcrB (for modified cytosine restriction). Since 5-methylcytosine containing DNA is widespread in nature, the Mcr systems probably have a broad biological role. Mcr restriction may seriously interfere with molecular cloning of 5-methylcytosine-containing foreign DNAs. The Mcr phenotypes of some commonly used strains of E. coli K-12 are reported.
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Cited by
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Metadata
- Authors
- Raleigh, E. A., & Wilson, G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-12
- Volume
- 83
- Issue
- 23
- Pages
- 9070-9074
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2073287325
- Cited by
- 316
- References
- 0
- FWCI
- 17.89
- Type
- journal article
- Language
- en
- Open access
- green
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