The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Isolation of cDNA clones coding for the catalytic subunit of mouse cAMP-dependent protein kinase.
Open accessAbstract
mRNA coding for the catalytic (C) subunit of cAMP-dependent protein kinase (ATP: protein phosphotransferase, EC 2.7.1.37) was partially purified from bovine testis by polysome immunoadsorption and oligo(dT)-chromatography. This enriched mRNA preparation was used to prepare and differentially screen a cDNA library. One of the selected cDNA clones was shown to hybrid-select mRNA coding for a 40-kDa protein that was specifically precipitated with antibodies to the C subunit. This bovine cDNA clone was then used to isolate a series of mouse cDNA clones that are complementary to the entire mouse C subunit mRNA. The mouse clones code for a protein of 351 amino acids that shows 98% homology to the bovine C subunit and hybridize to a single mRNA of 2.4 kilobases in mouse heart and brain. Southern blot analysis of total genomic DNA suggests that there is a single mouse gene coding for the C subunit. mRNA levels for both the C subunit and the type I regulatory subunit in various mouse tissues and cell lines were quantitated and compared by using single-stranded RNA probes prepared with SP6 polymerase.
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Cites
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Cited by
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Atomic structure of the MAP kinase ERK2 at 2.3 A resolution
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CYCLIC AMP SIGNALING AND GENE REGULATION
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Metadata
- Authors
- Uhler, M. D., Carmichael, D. F., Lee, D. C., Chrivia, J. C., Krebs, E. G., & McKnight, G. S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-03
- Volume
- 83
- Issue
- 5
- Pages
- 1300-1304
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1969994632
- Cited by
- 271
- References
- 26
- FWCI
- 8.43
- Type
- journal article
- Language
- en
- Open access
- green
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- Uhler, M. D., Carmichael, D. F., Lee, D. C., Chrivia, J. C., Krebs, E. G., & McKnight, G. S. (1986). Isolation of cDNA clones coding for the catalytic subunit of mouse cAMP-dependent protein kinase. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.5.1300
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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