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Characterization of sgk, a Novel Member of the Serine/Threonine Protein Kinase Gene Family Which Is Transcriptionally Induced by Glucocorticoids and Serum
Open accessAbstract
A novel member of the serine/threonine protein kinase gene family, designated sgk, for serum and glucocorticoid-regulated kinase, was identified in a differential screen for glucocorticoid-inducible transcripts expressed in the Con8.hd6 rat mammary tumor cell line. sgk encodes a protein of 49 kDa which has significant sequence homology (45 to 55% identity) throughout its catalytic domain with rac protein kinase, the protein kinase C family, ribosomal protein S6 kinase, and cyclic AMP-dependent protein kinase. sgk mRNA is expressed in most adult rat tissues, with the highest levels in the thymus, ovary, and lung, as well as in several rodent and human cell lines. sgk mRNA was stimulated by glucocorticoids and by serum within 30 min, and both inductions were independent of de novo protein synthesis. The transcriptional regulation by glucocorticoids is a primary response, since the promoter of sgk contains a glucocorticoid response element consensus sequence 1.0 kb upstream of the start of transcription which is able to stimulate chloramphenicol acetyltransferase reporter gene activity in a dexamethasone-dependent manner. Antibodies that specifically recognize sgk-encoded protein on an immunoblot were generated. This protein was shown to increase in abundance with glucocorticoid treatment in a manner which paralleled the mRNA accumulation. This is the first report of a presumed serine/threonine protein kinase that is highly regulated at the transcriptional level by glucocorticoid hormones and suggests a novel interplay between glucocorticoid receptor signalling and a protein kinase of the second messenger family.
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SGK1 is a serine/threonine kinase that is extensively expressed in most tissues.
“Serum/Glucocorticoid Regulated Kinase 1 (SGK1), a member of the AGC kinase family (cAMP-dependent, cGMP-dependent, and protein kinase C), is a serine/threonine kinase that exhibits extensive expression in a majority of tissues”
From Serum/glucocorticoid regulated kinase 1 (SGK1) in neurological disorders: pain or gain (2024) · cites this paper as Webster et al., 1993
The paper describes sgk as a member of the serine/threonine protein kinase gene family and reports that its mRNA is expressed in most adult rat tissues (opening summary; page not provided). The claim broadens that finding to SGK1 expression in most tissues generally, whereas the cited evidence concerns mRNA in adult rats. (LLM, conf=0.86)
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Metadata
- Authors
- Webster, M. K., Goya, L., Ge, Y., Maiyar, A. C., & Firestone, G. L.
- Affiliations
- University of California, Berkeley (US)
- ORCID
- Goya, Luis
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1993-04-01
- Volume
- 13
- Issue
- 4
- Pages
- 2031-2040
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC359524
- OpenAlex
- W2206511590
- Cited by
- 581
- References
- 4
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 6 · 2023: 21 · 2022: 14 · 2021: 16 · 2020: 8 · 2019: 15 · 2018: 15 · 2017: 14 · 2016: 18 · 2015: 14 · 2014: 12 · 2013: 15 · 2012: 23
- FWCI
- 3.38
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5p01ca005388-36)
- U.S. Public Health Service
- National Cancer Institute (CA 09041, CA-05388)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- serum and glucocorticoid-regulated kinase, SGK, serine/threonine kinase, glucocorticoid response element, glucocorticoid induction, serum induction, transcriptional regulation, S6K, protein kinase A, dexamethasone, tissue distribution
- MeSH terms
- Serum-Glucocorticoid Regulated Kinases, Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA, Glucocorticoids, Molecular Sequence Data, Nuclear Proteins, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid, RNA, Messenger, Transcription, Genetic, Restriction Mapping, Gene Library, Gene Expression, Consensus Sequence, Sequence Alignment, Protein Serine-Threonine Kinases, Immediate-Early Proteins, Mice, In Vitro Techniques
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