The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA.
Open accessAbstract
The hepatoblastoma cell line Hep G2 was transfected with a plasmid carrying the gene that confers resistance to G418 and four 5'-3' tandem copies of the hepatitis B virus (HBV) genome positioned such that two dimers of the genomic DNA are 3'-3' with respect to one another. Cells of one clone that grew in the presence of G418 produce high levels of hepatitis B e antigen and of hepatitis B surface antigen. HBV DNA is carried by these cells as chromosomally integrated sequences and episomally as relaxed circular, covalently closed, and incomplete copies of the HBV genome. Viral DNA was detected also in conditioned growth medium at the buoyant densities characteristic for infectious Dane and immature core particles. Finally, HBV-specific components morphologically identical to the 22-nm spherical and filamentous hepatitis B surface antigen particles as well as 42-nm Dane particles were visualized by immunoelectron microscopic analysis. Therefore, we have demonstrated that the Hep G2 cell line can support the assembly and secretion not only of several of the replicative intermediates of HBV DNA but also of Dane-like particles. This in vitro system can now be used to study the life cycle of HBV and the reaction of immunocompetent cells with cells carrying HBV.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Selective extraction of polyoma DNA from infected mouse cell cultures
Hirt, B. (1967) · Journal of Molecular Biology · doi:10.1016/0022-2836(67)90307-5 · Cited by 5,146
Knowles, B. B., Howe, C. C., & Aden, D. P. (1980) · Science · doi:10.1126/science.6248960 · Cited by 2,025
Rapid transfer of DNA from agarose gels to nylon membranes
Reed, K. C., & Mann, D. A. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.20.7207 · Cited by 1,761
Summers, J., & Mason, W. S. (1982) · Cell · doi:10.1016/0092-8674(82)90157-x · Cited by 1,443
A physical map of the DNA regions flanking the rabbit β-globin gene
Jeffreys, A. (1977) · Cell · doi:10.1016/0092-8674(77)90119-2 · Cited by 644
New procedure for DNA transfection with polycation and dimethyl sulfoxide.
Kawai, S., & Nishizawa, M. (1984) · Molecular and Cellular Biology · doi:10.1128/mcb.4.6.1172 · Cited by 494
Ou, J.-H. J., Laub, O., & Rutter, W. J. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.6.1578 · Cited by 340
Büscher, M., Reiser, W., Will, H., & Schaller, H. (1985) · Cell · doi:10.1016/0092-8674(85)90220-x · Cited by 141
Hepatitis B virus DNA forms in nuclear and cytoplasmic fractions of infected human liver
Miller, R. H., & Robinson, W. S. (1984) · Virology · doi:10.1016/0042-6822(84)90231-9 · Cited by 93
Yoakum, G. H., Korba, B. E., Lechner, J. F., Tokiwa, T., Gazdar, A. F., Seeley, T. W., Siegel, M., Leeman, L., Autrup, H. N., & Harris, C. C. (1983) · Science · doi:10.1126/science.6194563 · Cited by 83
Cited by
Ladner, S. K., Otto, M. J., Barker, C. S., Zaifert, K., Wang, G. H., Guo, J. T., Seeger, C., & King, R. W. (1997) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.41.8.1715 · Cited by 640
Inhibition of hepatitis B virus in mice by RNA interference
McCaffrey, A. P., Nakai, H., Pandey, K., Huang, Z., Salazar, F. H., Xu, H., Wieland, S. F., Marion, P. L., & Kay, M. A. (2003) · Nature Biotechnology · doi:10.1038/nbt824 · Cited by 607
Inhibition of Hepatitis B Virus Replication by Drug-Induced Depletion of Nucleocapsids
Deres, K., Schröder, C. H., Paessens, A., Goldmann, S., Hacker, H. J., Weber, O., Krämer, T., Niewöhner, U., Pleiss, U., Stoltefuss, J., et al. (2003) · Science · doi:10.1126/science.1077215 · Cited by 508
Hepatitis B virus X protein is essential to initiate and maintain virus replication after infection
Lucifora, J., Arzberger, S., Durantel, D., Belloni, L., Strubin, M., Levrero, M., Zoulim, F., Hantz, O., & Protzer, U. (2011) · Journal of Hepatology · doi:10.1016/j.jhep.2011.02.015 · Cited by 481
Shih, C. M., Lo, S. J., Miyamura, T., Chen, S. Y., & Lee, Y. H. (1993) · Journal of Virology · doi:10.1128/jvi.67.10.5823-5832.1993 · Cited by 332
Metadata
- Authors
- Sells, M. A., Chen, M. L., & Acs, G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1987-02
- Volume
- 84
- Issue
- 4
- Pages
- 1005-1009
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2010338556
- Cited by
- 1,117
- References
- 12
- FWCI
- 23.06
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA. https://app.cochat.ai/papers/10.1073/pnas.84.4.1005
- The paper
- Sells, M. A., Chen, M. L., & Acs, G. (1987). Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.84.4.1005
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
