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Ubiquitin is detected in neurofibrillary tangles and senile plaque neurites of Alzheimer disease brains.
Open accessAbstract
Neurofibrillary tangles (NFT) and neurites associated with senile plaques (SP) in Alzheimer disease-affected brain tissues were specifically immunostained with affinity-purified antibody preparations directed against ubiquitin. In addition, a class of neurites seen in brain regions containing NFT and SP were also specifically stained. Cross-reactivity of the ubiquitin antisera for tau protein, neurofilament proteins, and high molecular weight microtubule-associated proteins (MAPs) were ruled out by (i) the inability of the ubiquitin antisera to stain these proteins in immunoblotting experiments and (ii) the inability of tau, neurofilament, and MAP preparations, when preincubated with the ubiquitin antisera, to inhibit the selective neurofibrillar staining observed. Our results are consistent with the suggestion that ubiquitin is covalently associated with the insoluble neurofibrillary material of NFT and SP. We propose that the ubiquitin-mediated degradative pathway may be ineffective in removing these fibrillar structures in Alzheimer disease brain.
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Metadata
- Authors
- Perry, G., Friedman, R., Shaw, G., & Chau, V
- Affiliations
- Case Western Reserve University (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1987-05
- Volume
- 84
- Issue
- 9
- Pages
- 3033-3036
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC304795
- OpenAlex
- W2046720254
- Cited by
- 623
- References
- 27
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 11 · 2023: 13 · 2022: 8 · 2021: 15 · 2020: 17 · 2019: 15 · 2018: 21 · 2017: 11 · 2016: 17 · 2015: 15 · 2014: 5 · 2013: 20 · 2012: 18
- FWCI
- 33.38
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute on Aging (AG00795)
- National Institute of General Medical Sciences (GM35803)
- National Institute of Neurological Disorders and Stroke (NS22695)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Keywords
- ubiquitin, neurofibrillary tangles, amyloid plaques, Alzheimer's disease, neurites, protein degradation, tau protein, neurofilaments, microtubule-associated proteins, immunostaining
- MeSH terms
- Aged, Alzheimer Disease, Antibodies, Antigen-Antibody Complex, Axons, Brain, Humans, Immunoenzyme Techniques, Neurons, Ubiquitins
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