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Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.
Open accessAbstract
An alpha 2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet alpha 2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet alpha 2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the alpha 2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the alpha 2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet alpha 2-adrenergic receptor (alpha 2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective alpha-adrenergic ligands.
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Citations
Cited by
Arnsten, A. F. T. (1996) · Archives of General Psychiatry · doi:10.1001/archpsyc.1996.01830050084013 · Cited by 458
Historical review: A brief history and personal retrospective of seven-transmembrane receptors
Lefkowitz, R. J. (2004) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2004.06.006 · Cited by 417
Self-citationGene targeting — homing in on α2-adrenoceptor-subtype function
MacDonald, E., Kobilka, B. K., & Scheinin, M. (1997) · Trends in Pharmacological Sciences · doi:10.1016/s0165-6147(97)01063-8 · Cited by 310
Self-citationHorstman, D. A., Brandon, S., Wilson, A. L., Guyer, C. A., Cragoe, E. J., & Limbird, L. E. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)45781-x · Cited by 271
Structure, function, and regulation of adrenergic receptors
Strosberg, A. D. (1993) · Protein Science · doi:10.1002/pro.5560020802 · Cited by 248
Metadata
- Authors
- Regan, J. W., Kobilka, T. S., Yang-Feng, T. L., Caron, M. G., Lefkowitz, R. J., & Kobilka, B. K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1988-09
- Volume
- 85
- Issue
- 17
- Pages
- 6301-6305
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2009133356
- Cited by
- 434
- References
- 0
- FWCI
- 15.88
- Type
- journal article
- Language
- en
- Open access
- green
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- Regan, J. W., Kobilka, T. S., Yang-Feng, T. L., Caron, M. G., Lefkowitz, R. J., & Kobilka, B. K. (1988). Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.85.17.6301
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