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Cited by 615Immunotherapy and Immune Responsesvaccines and immunoinformatics approachesT-cell and B-cell Immunology

Phagocytic processing of bacterial antigens for class I MHC presentation to T cells

Pfeifer, John D., Wick, Mary Jo, Roberts, Richard L., Findlay, Kirk, Normark, Staffan J., Harding, Clifford V.·1993·Nature
DOI 10.1038/361359a0
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Abstract

Class I major histocompatibility complex (MHC) molecules present antigens that are produced within the presenting cell or penetrate from the vacuolar system into the cytosol for processing. Most studies of exogenous antigen processing have used soluble antigens, which are not efficiently presented by class I MHC molecules and do not elicit CD8 T-cell responses in vivo. But particulate antigen preparations with no known mechanism for cytosolic penetration can also elicit CD8 T-cell responses in vivo. We report here that phagocytosis of bacteria with no mechanism for cytosolic penetration also results in presentation of bacterial antigens by class I MHC molecules. Moreover, this mechanism is resistant to cycloheximide and Brefeldin A, which block the classical class I processing pathway. These results suggest a novel vacuolar class I processing pathway for exogenous phagocytic antigens.

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Crossref · 10.1038/361359a0

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  • Melanoma cells and antigen-presenting cells can both display HLA-I and HLA-II peptides of bacterial origin.

    “We demonstrate that bacterial HLA-I and HLA-II peptides can be presented by both antigen-presenting cells (as has previously been shown25–28) and by melanoma cells”

    From Identification of bacteria-derived HLA-bound peptides in melanoma (2021) · cites this paper as [26]

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Authors
Pfeifer, J. D., Wick, M. J., Roberts, R. L., Findlay, K., Normark, S. J., & Harding, C. V.
Affiliations
Washington University in St. Louis (US)
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
1993-01
Volume
361
Issue
6410
Pages
359-362
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/361359a0
OpenAlex
W2042470561
Cited by
615
References
31
Citations by year
2026: 2 · 2025: 3 · 2024: 2 · 2023: 7 · 2022: 3 · 2021: 6 · 2020: 8 · 2019: 6 · 2018: 8 · 2017: 9 · 2015: 4 · 2014: 8 · 2013: 10 · 2012: 10
FWCI
22.81
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Keywords
MHC class I, antigen processing, phagocytosis, bacterial antigens, CD8+ T cells, exogenous antigen presentation, cycloheximide resistance, particulate antigen
MeSH terms
Amino Acid Sequence, Animals, Epitopes, Antigens, Bacterial, Bacterial Proteins, Escherichia coli, Macrophages, Mice, Inbred CBA, Mice, Inbred C57BL, Microscopy, Electron, Molecular Sequence Data, Ovalbumin, Phagocytosis, Recombinant Fusion Proteins, Salmonella typhimurium, T-Lymphocytes, Vacuoles, Histocompatibility Antigens Class I, T-Lymphocyte Subsets, CD8 Antigens, Escherichia coli Proteins, Mice

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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

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