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Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
Open accessAbstract
Maturation-promoting factor causes germinal vesicle breakdown when injected into Xenopus oocytes and can induce metaphase in a cell-free system. The cell-free assay was used to monitor maturation-promoting factor during its purification from unfertilized Xenopus eggs. Ammonium sulfate precipitation and six chromatographic procedures resulted in a preparation purified greater than 3000-fold that could induce germinal vesicle breakdown within 2 hr when injected into cycloheximide-treated oocytes. Proteins of 45 kDa and 32 kDa were correlated with fractions of highest activity in both assays. These fractions contained a protein kinase activity able to phosphorylate the endogenous 45-kDa protein, as well as histone H1, phosphatase inhibitor 1, and casein. The highly purified preparations described here should help to identify the mechanism of action of maturation-promoting factor and to elucidate the role of protein kinases in the induction of metaphase.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Cited by
A quantitative atlas of mitotic phosphorylation
Dephoure, N., Zhou, C., Villén, J., Beausoleil, S. A., Bakalarski, C. E., Elledge, S. J., & Gygi, S. P. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0805139105 · Cited by 1,608
Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs
Verde, F., Labbé, J.-C., Dorée, M., & Karsenti, E. (1990) · Nature · doi:10.1038/343233a0 · Cited by 441
Mendenhall, M. D., & Hodge, A. E. (1998) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.62.4.1191-1243.1998 · Cited by 429
Diril, M. K., Ratnacaram, C. K., Padmakumar, V. C., Du, T., Wasser, M., Coppola, V., Tessarollo, L., & Kaldis, P. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1115201109 · Cited by 385
From Cdc2 to Cdk1: when did the cell cycle kinase join its cyclin partner?
Dorée, M., & Hunt, T. (2002) · Journal of Cell Science · doi:10.1242/jcs.115.12.2461 · Cited by 274
Metadata
- Authors
- Lohka, M. J., Hayes, M. K., & Maller, J. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1988-05
- Volume
- 85
- Issue
- 9
- Pages
- 3009-3013
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2015150670
- Cited by
- 644
- References
- 1
- FWCI
- 25.10
- Type
- journal article
- Language
- en
- Open access
- green
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- Lohka, M. J., Hayes, M. K., & Maller, J. L. (1988). Purification of maturation-promoting factor, an intracellular regulator of early mitotic events. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.85.9.3009
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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