The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I.
Open accessAbstract
Incorporation of dideoxynucleotides by T7 DNA polymerase and Escherichia coli DNA polymerase I is more efficient when Mn2+ rather than Mg2+ is used for catalysis. Substituting Mn2+ for Mg2+ reduces the discrimination against dideoxynucleotides approximately 100-fold for DNA polymerase I and 4-fold for T7 DNA polymerase. With T7 DNA polymerase and Mn2+, dideoxynucleotides and deoxynucleotides are incorporated at virtually the same rate. Mn2+ also reduces the discrimination against other analogs with modifications in the furanose moiety, the base, and the phosphate linkage. A metal buffer, isocitrate, expands the MnCl2 concentration range effective in catalyzing DNA synthesis. The lack of discrimination against dideoxynucleoside triphosphates using T7 DNA polymerase and Mn2+ results in uniform terminations of DNA sequencing reactions, with the intensity of adjacent bands on polyacrylamide gels varying in most instances by less than 10%.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Principles of Nucleic Acid Structure
(2008) · Elsevier eBooks · doi:10.1016/b978-0-12-369507-9.x5001-8 · Cited by 4,504
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
Tabor, S., & Richardson, C. C. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.14.4767 · Cited by 2,241
Self-citationFluorescence detection in automated DNA sequence analysis
Smith, L. M., Sanders, J. Z., Kaiser, R. J., Hughes, P., Dodd, C., Connell, C. R., Heiner, C., Kent, S. B. H., & Hood, L. E. (1986) · Nature · doi:10.1038/321674a0 · Cited by 1,714
Principles of Nucleic Acid Structure
Parish, J. (1985) · Biochemical Education · doi:10.1016/0307-4412(85)90046-9 · Cited by 1,486
A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides
Prober, J. M., Trainor, G. L., Dam, R. J., Hobbs, F. W., Robertson, C. W., Zagursky, R. J., Cocuzza, A. J., Jensen, M. A., & Baumeister, K. (1987) · Science · doi:10.1126/science.2443975 · Cited by 855
Tabor, S., Huber, H. E., & Richardson, C. C. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47718-6 · Cited by 405
Self-citationVogel, H. J., Bryson, V., & Lampen, J. O. (1962) · Science · doi:10.1126/science.138.3543.912 · Cited by 368
Enzymatic Synthesis of Deoxyribonucleic Acid
Englund, P. T., Huberman, J. A., Jovin, T. M., & Kornberg, A. (1969) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)91726-6 · Cited by 272
Tabor, S., & Richardson, C. C. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)83369-5 · Cited by 255
Self-citation
Cited by
Making and Breaking Nucleic Acids: Two-Mg2+-Ion Catalysis and Substrate Specificity
Yang, W., Lee, J. Y., & Nowotny, M. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.03.013 · Cited by 573
Bile Salt Hydrolase of Bifidobacterium longum —Biochemical and Genetic Characterization
Tanaka, H., Hashiba, H., Kok, J., & Mierau, I. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.6.2502-2512.2000 · Cited by 285
Arnold, J. J., & Cameron, C. E. (2004) · Biochemistry · doi:10.1021/bi035212y · Cited by 178
Smith, R. L., Thompson, L. J., & Maguire, M. E. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.5.1233-1238.1995 · Cited by 127
Multiple prebiotic metals mediate translation
Bray, M. S., Lenz, T. K., Haynes, J. W., Bowman, J. C., Petrov, A. S., Reddi, A. R., Hud, N. V., Williams, L. D., & Glass, J. B. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1803636115 · Cited by 58
Metadata
- Authors
- Tabor, S., & Richardson, C. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-06
- Volume
- 86
- Issue
- 11
- Pages
- 4076-4080
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2026491313
- Cited by
- 288
- References
- 19
- FWCI
- 4.84
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I. https://app.cochat.ai/papers/10.1073/pnas.86.11.4076
- The paper
- Tabor, S., & Richardson, C. C. (1989). Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.11.4076
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
