The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Horizontal transfer of penicillin-binding protein genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae.
Open accessAbstract
Resistance to penicillin in clinical isolates of Streptococcus pneumoniae has occurred by the development of altered penicillin-binding proteins (PBPs) that have greatly decreased affinity for the antibiotic. We have investigated the origins of penicillin-resistant strains by comparing the sequences of the transpeptidase domain of PBP2B from 6 penicillin-sensitive and 14 penicillin-resistant strains. In addition we have sequenced part of the amylomaltase gene from 2 of the sensitive and 6 of the resistant strains. The sequences of the amylomaltase gene of all of the strains and of the PBP2B gene of the penicillin-sensitive strain show that S. pneumoniae is genetically very uniform. In contrast the PBP2B genes of the penicillin-resistant strains show approximately equal to 14% sequence divergence from those of the penicillin-sensitive strains and the development of penicillin resistance has involved the replacement, presumably by transformation, of the original PBP2B gene by a homologous gene from an unknown source. This genetic event has occurred on at least two occasions, involving different sources, to produce the two classes of altered PBP2B genes found in penicillin-resistant strains of S. pneumoniae. There is considerable variation among the PBP2B genes of the resistant strains that may have arisen by secondary transformation events accompanied by mismatch repair subsequent to their original introductions into S. pneumoniae.
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Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Cited by
Hybridization as an invasion of the genome
Mallet, J. (2005) · Trends in Ecology & Evolution · doi:10.1016/j.tree.2005.02.010 · Cited by 2,196
Diversity of PspA: Mosaic Genes and Evidence for Past Recombination in Streptococcus pneumoniae
Hollingshead, S. K., Becker, R., & Briles, D. E. (2000) · Infection and Immunity · doi:10.1128/iai.68.10.5889-5900.2000 · Cited by 339
Antibiotic Resistance in Streptococcus pneumoniae
Tomasz, A. (1997) · Clinical Infectious Diseases · doi:10.1093/clinids/24.supplement_1.s85 · Cited by 264
Self-citationMolecular Mechanisms of β-Lactam Resistance in Streptococcus Pneumoniae
Hakenbeck, R., Brückner, R., Denapaite, D., & Maurer, P. (2012) · Future Microbiology · doi:10.2217/fmb.12.2 · Cited by 183
Metadata
- Authors
- Dowson, C. G., Hutchison, A., Brannigan, J. A., George, R. C., Hansman, D., Liñares, J., Tomasz, A., Smith, J. M., & Spratt, B. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-11
- Volume
- 86
- Issue
- 22
- Pages
- 8842-8846
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2042859316
- Cited by
- 404
- References
- 1
- FWCI
- 10.58
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Horizontal transfer of penicillin-binding protein genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae. https://app.cochat.ai/papers/10.1073/pnas.86.22.8842
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- Dowson, C. G., Hutchison, A., Brannigan, J. A., George, R. C., Hansman, D., Liñares, J., Tomasz, A., Smith, J. M., & Spratt, B. G. (1989). Horizontal transfer of penicillin-binding protein genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.22.8842
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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