The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels.
Open accessAbstract
We have subverted a receptor-mediated endocytosis event to transport genes into human leukemic cells. By coupling the natural iron-delivery protein transferrin to the DNA-binding polycations polylysine or protamine, we have created protein conjugates that bind nucleic acids and carry them into the cell during the normal transferrin cycle [Wagner, E., Zenke, M., Cotten, M., Beug, H. & Birnstiel, M. L. (1990) Proc. Natl. Acad. Sci. USA 87, 3410-3414]. We demonstrate here that this procedure is useful for a human leukemic cell line. We enhanced the rate of gene delivery by (i) increasing the transferrin receptor density through treatment of the cells with the cell-permeable iron chelator desferrioxamine, (ii) interfering with the synthesis of heme with succinyl acetone treatment, or (iii) stimulating the degradation of heme with cobalt chloride treatment. Consistent with gene delivery as an endocytosis event, we show that the subsequent expression in K-562 cells of a gene included in the transported DNA depends upon the cellular presence of the lysosomotropic agent chloroquine. By contrast, monensin blocks "transferrinfection," as does incubation of the cells at 18 degrees C.
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Cites
Firefly luciferase gene: structure and expression in mammalian cells.
de Wet, J. R., Wood, K. V., DeLuca, M., Helinski, D. R., & Subramani, S. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.2.725 · Cited by 3,087
Harding, C., Heuser, J., & Stahl, P. (1983) · The Journal of Cell Biology · doi:10.1083/jcb.97.2.329 · Cited by 1,775
Perturbation of vesicular traffic with the carboxylic ionophore monensin
Tartakoff, A. M. (1983) · Cell · doi:10.1016/0092-8674(83)90286-6 · Cited by 873
Receptor-mediated in vitro gene transformation by a soluble DNA carrier system.
Wu, G. Y., & Wu, C. H. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61209-8 · Cited by 855
High efficiency polyoma DNA transfection of chloroquine treated cells
Luthman, H., & Magnusson, G. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.5.1295 · Cited by 677
Transferrin-polycation conjugates as carriers for DNA uptake into cells.
Wagner, E., Zenke, M., Cotten, M., Beug, H., & Birnstiel, M. L. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.9.3410 · Cited by 655
Self-citationReceptor-mediated gene delivery and expression in vivo.
Wu, G. Y., & Wu, C. H. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68081-0 · Cited by 635
The physiology of transferrin and transferrin receptors.
Huebers, H. A., & Finch, C. A. (1987) · Physiological Reviews · doi:10.1152/physrev.1987.67.2.520 · Cited by 586
Developmental regulation of β-globin gene switching
Choi, O.-R. B., & Engel, J. D. (1988) · Cell · doi:10.1016/0092-8674(88)90005-0 · Cited by 325
Transferrin and transferrin receptors in carcinoma of the breast
Page Faulk, W., Hsi, B.-L., & Stevens, P. J. (1980) · The Lancet · doi:10.1016/s0140-6736(80)90440-7 · Cited by 280
Cited by
Design and development of polymers for gene delivery
Pack, D. W., Hoffman, A. S., Pun, S., & Stayton, P. S. (2005) · Nature Reviews Drug Discovery · doi:10.1038/nrd1775 · Cited by 2,568
Polyamidoamine cascade polymers mediate efficient transfection of cells in culture
Haensler, J., & Szoka, F. C. (1993) · Bioconjugate Chemistry · doi:10.1021/bc00023a012 · Cited by 1,207
Targeted Drug Delivery via the Transferrin Receptor-Mediated Endocytosis Pathway
Qian, Z. M., Li, H., Sun, H., & Ho, K. (2002) · Pharmacological Reviews · doi:10.1124/pr.54.4.561 · Cited by 1,019
The transferrin receptor and the targeted delivery of therapeutic agents against cancer
Daniels, T. R., Bernabeu, E., Rodríguez, J. A., Patel, S., Kozman, M., Chiappetta, D. A., Holler, E., Ljubimova, J. Y., Helguera, G., & Penichet, M. L. (2012) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2011.07.016 · Cited by 807
Wagner, E., Plank, C., Zatloukal, K., Cotten, M., & Birnstiel, M. L. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.17.7934 · Cited by 658
Self-citation
Metadata
- Authors
- Cotten, M., Längle-Rouault, F., Kirlappos, H., Wagner, E., Mechtler, K., Zenke, M., Beug, H., & Birnstiel, M. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-06
- Volume
- 87
- Issue
- 11
- Pages
- 4033-4037
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2022576626
- Cited by
- 337
- References
- 25
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels. https://app.cochat.ai/papers/10.1073/pnas.87.11.4033
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- Cotten, M., Längle-Rouault, F., Kirlappos, H., Wagner, E., Mechtler, K., Zenke, M., Beug, H., & Birnstiel, M. L. (1990). Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.87.11.4033
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
