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Amplified RNA synthesized from limited quantities of heterogeneous cDNA.
Open accessAbstract
The heterogeneity of neural gene expression and the spatially limited expression of many low-abundance messenger RNAs in the brain has made cloning and analysis of such messages difficult. To generate amounts of nucleic acids sufficient for use in standard cloning strategies, we have devised a method for producing amplified heterogeneous populations of RNA from limited quantities of cDNA. Whole cerebellar RNA was primed with a synthetic oligonucleotide containing the T7 RNA polymerase promoter sequence 5' to a polythymidylate region. After second-strand cDNA synthesis, T7 RNA polymerase was used to generate amplified antisense RNA (aRNA). Up to 80-fold molar amplification has been achieved from nanogram quantities of cDNA. The amplified material is similar in size distribution to the parent cDNA and shows sequence heterogeneity as assessed by Southern and Northern blot analysis. Specific messages for moderate-abundance mRNAs for actin and guanine nucleotide-binding protein (G-protein) alpha subunits have been detected in the amplified material. By using in situ transcription to generate cDNA, sequences for cyclophilin have been detected in aRNA derived from single cerebellar tissue sections. cDNA derived from a single cerebellar Purkinje cell also has been amplified and yields material that hybridizes to cognate whole RNA and mRNA but not to Escherichia coli RNA.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Cited by
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Ståhl, P. L., Salmén, F., Vickovic, S., Lundmark, A., Navarro, J. F., Magnusson, J., Giacomello, S., Asp, M., Westholm, J. O., Huss, M., et al. (2016) · Science · doi:10.1126/science.aaf2403 · Cited by 3,834
Regev, A., Teichmann, S. A., Lander, E. S., Amit, I., Benoist, C., Birney, E., Bodenmiller, B., Campbell, P., Carninci, P., Clatworthy, M., et al. (2017) · eLife · doi:10.7554/elife.27041 · Cited by 2,232
Self-citationGeiger, J. R. P., Melcher, T., Koh, D.-S., Sakmann, B., Seeburg, P. H., Jonas, P., & Monyer, H. (1995) · Neuron · doi:10.1016/0896-6273(95)90076-4 · Cited by 1,260
Museum of spatial transcriptomics
Moses, L., & Pachter, L. (2022) · Nature Methods · doi:10.1038/s41592-022-01409-2 · Cited by 1,255
Control of Kinetic Properties of AMPA Receptor Channels by Nuclear RNA Editing
Lomeli, H., Mosbacher, J., Melcher, T., Höger, T., Geiger, J. R. P., Kuner, T., Monyer, H., Higuchi, M., Bach, A., & Seeburg, P. H. (1994) · Science · doi:10.1126/science.7992055 · Cited by 746
Coordinated Expression of Dopamine Receptors in Neostriatal Medium Spiny Neurons
Surmeier, D. J., Song, W.-J., & Yan, Z. (1996) · The Journal of Neuroscience · doi:10.1523/jneurosci.16-20-06579.1996 · Cited by 747
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Gaucher, D., Therrien, R., Kettaf, N., Angermann, B. R., Boucher, G., Filali-Mouhim, A., Moser, J. M., Mehta, R. S., Drake, D. R., Castro, E., et al. (2008) · The Journal of Experimental Medicine · doi:10.1084/jem.20082292 · Cited by 594
Gritsko, T., Williams, A., Turkson, J., Kaneko, S., Bowman, T., Huang, M., Nam, S., Eweis, I., Diaz, N., Sullivan, D., et al. (2006) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-04-1752 · Cited by 563
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Yan, W. X., Mirzazadeh, R., Garnerone, S., Scott, D., Schneider, M. W., Kallas, T., Custodio, J., Wernersson, E., Li, Y., Gao, L., et al. (2017) · Nature Communications · doi:10.1038/ncomms15058 · Cited by 369
“The t(x) is modelled using a third-order B-spline, a continuously differentiable function defined on interval (1,20) and a g ð Þ 2 R(1).”
MethodEschrich, S. A., Pramana, J., Zhang, H., Zhao, H., Boulware, D., Lee, J.-H., Bloom, G., Rocha-Lima, C., Kelley, S., Calvin, D. P., et al. (2009) · International Journal of Radiation Oncology*Biology*Physics · doi:10.1016/j.ijrobp.2009.06.014 · Cited by 360
Altar, C. A., Jurata, L. W., Charles, V., Lemire, A., Liu, P., Bukhman, Y., Young, T. A., Bullard, J., Yokoe, H., Webster, M. J., et al. (2005) · Biological Psychiatry · doi:10.1016/j.biopsych.2005.03.031 · Cited by 318
Burgdorf, J., Zhang, X.-L., Nicholson, K. L., Balster, R. L., David Leander, J., Stanton, P. K., Gross, A. L., Kroes, R. A., & Moskal, J. R. (2013) · Neuropsychopharmacology · doi:10.1038/npp.2012.246 · Cited by 284
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Jazaeri, A. A., Awtrey, C. S., Chandramouli, G. V. R., Chuang, Y. E., Khan, J., Sotiriou, C., Aprelikova, O., Yee, C. J., Zorn, K. K., Birrer, M. J., et al. (2005) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-04-2682 · Cited by 192
Molecular basis for catecholaminergic neuron diversity
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PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
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“It must be pointed out that only ethanol precipitation could be utilized for methods based on the classical Eberwine approach (38), since it requires RNA priming for DNA synthesis.”
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Self-citationDuong, C., Greenawalt, D. M., Kowalczyk, A., Ciavarella, M. L., Raskutti, G., Murray, W. K., Phillips, W. A., & Thomas, R. J. S. (2007) · Annals of Surgical Oncology · doi:10.1245/s10434-007-9550-1 · Cited by 64
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Backes, E., & Hemby, S. E. (2003) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.103.054965 · Cited by 48
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Buss, N. A. P. S., Gavins, F. N. E., Cover, P. O., Terron, A., & Buckingham, J. C. (2015) · The FASEB Journal · doi:10.1096/fj.14-268375 · Cited by 23
Metadata
- Authors
- Van Gelder, R. N., von Zastrow, M. E., Yool, A., Dement, W. C., Barchas, J. D., & Eberwine, J. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-03
- Volume
- 87
- Issue
- 5
- Pages
- 1663-1667
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2121777852
- Cited by
- 1,262
- References
- 2
- FWCI
- 1.35
- Type
- journal article
- Language
- en
- Open access
- green
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