Low density lipoprotein receptor-binding activity in human tissues: quantitative importance of hepatic receptors and evidence for regulation of their expression in vivo.
Open accessAbstract
The heparin-sensitive binding of 125I-labeled low-density lipoprotein (LDL) to homogenates from 18 different normal human tissues and some solid tumors was determined. The binding to adrenal and liver homogenates fulfilled criteria established for the binding of LDL to its receptor--namely, (i) saturability, (ii) sensitivity to proteolytic destruction, (iii) inhibition by EDTA, and (iv) heat sensitivity. When the binding of 125I-labeled LDL was assayed at a constant concentration (50 micrograms/ml), the adrenal gland and the ovary had the highest binding of normal tissues. The highest binding per g of tissue overall was obtained in homogenates of a gastric carcinoma and a parotid adenoma. When the weights of the parenchymatous organs were considered, the major amount of LDL receptors was contained in the liver. To study the possible regulation of hepatic LDL-receptor expression, 11 patients were pretreated with cholestyramine (8 g twice a day for 3 weeks). Increased binding activity (+105%, P less than 0.001) was obtained in homogenates from liver biopsies from the cholestyramine-treated patients as compared with 12 untreated controls. It is concluded that the liver is the most important organ for LDL catabolism in humans and that the receptor activity in this organ can be regulated upon pharmacologic intervention. Further studies are needed to confirm the possibility that certain solid tumors can exhibit high numbers of LDL receptors.
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PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
A Receptor-Mediated Pathway for Cholesterol Homeostasis
Brown, M. S., & Goldstein, J. L. (1986) · Science · doi:10.1126/science.3513311 · Cited by 5,703 · see citing works
The Low-Density Lipoprotein Pathway and its Relation to Atherosclerosis
Goldstein, L. J., & Brown, S. M. (1977) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.46.070177.004341 · Cited by 2,455 · see citing works
Binding and Degradation of Low Density Lipoproteins by Cultured Human Fibroblasts
Goldstein, J. L., & Brown, M. S. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42341-7 · Cited by 1,485 · see citing works
Lipoprotein receptors and cholesterol homeostasis
Mahley, R. W., & Innerarity, T. L. (1983) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(83)90001-1 · Cited by 755 · see citing works
The metabolism of low density lipoprotein in familial type II hyperlipoproteinemia
Langer, T., Strober, W., & Levy, R. I. (1972) · Journal of Clinical Investigation · doi:10.1172/jci106949 · Cited by 632 · see citing works
Release of low density lipoprotein from its cell surface receptor by sulfated glycosaminoglycans
Goldstein, J. L., Basu, S. K., Brunschede, G. Y., & Brown, M. S. (1976) · Cell · doi:10.1016/0092-8674(76)90258-0 · Cited by 613 · see citing works
Lipoprotein receptors in the liver. Control signals for plasma cholesterol traffic.
Brown, M. S., & Goldstein, J. L. (1983) · Journal of Clinical Investigation · doi:10.1172/jci111044 · Cited by 545 · see citing works
Regulatory role for hepatic low density lipoprotein receptors in vivo in the dog.
Kovanen, P. T., Bilheimer, D. W., Goldstein, J. L., Jaramillo, J. J., & Brown, M. S. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.2.1194 · Cited by 421 · see citing works
Mahley, R. W., Hui, D. Y., Innerarity, T. L., & Weisgraber, K. H. (1981) · Journal of Clinical Investigation · doi:10.1172/jci110365 · Cited by 375 · see citing works
Cholestyramine Promotes Receptor-Mediated Low-Density-Lipoprotein Catabolism
Shepherd, J., Packard, C. J., Bicker, S., Lawrie, T. D. V., & Morgan, H. (1980) · New England Journal of Medicine · doi:10.1056/nejm198005293022202 · Cited by 356 · see citing works
Low-density lipoprotein (LDL) receptor activity in human acute myelogenous leukemia cells
Ho, Y.-K., Smith, R. G., Brown, M., et al. (1978) · Blood · doi:10.1182/blood.v52.6.1099.bloodjournal5261099 · Cited by 279 · see citing works
Familial Hypercholesterolemia: A Genetic Defect in the Low-Density Lipoprotein Receptor
Bleich, H. L., Boro, E. S., Brown, M. S., et al. (1976) · New England Journal of Medicine · doi:10.1056/nejm197606172942509 · Cited by 224 · see citing works
Goldstein, J. L., Helgeson, J., & Brown, M. S. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)50610-4 · Cited by 184 · see citing works
Kovanen, P. T., Basu, S. K., Goldstein, J. L., & Brown, M. S. (1979) · Endocrinology · doi:10.1210/endo-104-3-610 · Cited by 171 · see citing works
Elevated low density lipoprotein receptor activity in leukemic cells with monocytic differentiation
Vitols, S., Gahrton, G., Öst, Å., et al. (1984) · Blood · doi:10.1182/blood.v63.5.1186.1186 · Cited by 170 · see citing works
Review of lipid-lowering clinical trials in relation to observational epidemiologic studies.
Tyroler, H. (1987) · Circulation · doi:10.1161/01.cir.76.3.515 · Cited by 132 · see citing works
Spady, D. K., Meddings, J. B., & Dietschy, J. M. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112460 · Cited by 121 · see citing works
Angelin, B., Raviola, C. A., Innerarity, T. L., & Mahley, R. W. (1983) · Journal of Clinical Investigation · doi:10.1172/jci110835 · Cited by 120 · see citing works
Self-citationSelective delivery of cytotoxic compounds to cells by the LDL pathway
Firestone, R. A., Pisano, J. M., Falck, J. R., et al. (1984) · Journal of Medicinal Chemistry · doi:10.1021/jm00374a017 · Cited by 94 · see citing works
EPIDEMIOLOGY OF CARDIOVASCULAR DISEASES: CURRENT PERSPECTIVES
Kuller, L. H. (1976) · American Journal of Epidemiology · doi:10.1093/oxfordjournals.aje.a112315 · Cited by 90 · see citing works
Delivery of aclacinomycin A to human glioma cells in vitro by the low-density lipoprotein pathway.
Rudling, M. J., Collins, V. P., & Peterson, C. O. (1983) · PubMed · Cited by 78 · see citing works
Self-citationHepatic Catabolism of Low Density Lipoprotein: Mechanisms and Metabolic Consequences
Attie, A., Pittman, R. C., & Steinberg, D. H. (1982) · Hepatology · doi:10.1002/hep.1840020215 · Cited by 76 · see citing works
Targeted killing of cultured cells by receptor-dependent photosensitization.
Mosley, S. T., Goldstein, J. L., Brown, M. S., et al. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.9.5717 · Cited by 75 · see citing works
Hoeg, J. M., Demosky, S. J., Schaefer, E. J., Starzl, T. E., & Brewer, H. B. (1984) · Journal of Clinical Investigation · doi:10.1172/jci111229 · Cited by 64 · see citing works
Rudling, M., Ståhle, E., Peterson, C. O., et al. (1986) · BMJ · doi:10.1136/bmj.292.6520.580 · Cited by 62 · see citing works
Self-citationAngelin, B., Einarsson, K., Liljeqvist, L., et al. (1984) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34459-x · Cited by 58 · see citing works
Self-citationHarders-Spengel, K., Wood, C., Thompson, G. R., Myant, N. B., & Soutar, A. K. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.20.6355 · Cited by 56 · see citing works
Soutar, A. K., Harders-Spengel, K., Wade, D. P., et al. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)76008-6 · Cited by 50 · see citing works
Vitols, S., Masquelier, M., & Peterson, C. O. (1985) · Journal of Medicinal Chemistry · doi:10.1021/jm00382a011 · Cited by 41 · see citing works
Rudling, M. J., & Peterson, C. O. (1985) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(85)90225-5 · Cited by 34 · see citing works
Self-citationRudling, M. J., & Peterson, C. O. (1985) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(85)90091-8 · Cited by 29 · see citing works
Self-citationHoeg, J. M., Demosky, S. J., Lackner, K. J., Osborne, J. C., Oliver, C., & Brewer, H. B. (1986) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(86)90312-7 · Cited by 26 · see citing works
Meddings, J. B., Spady, D. K., & Dietschy, J. M. (1987) · American Heart Journal · doi:10.1016/0002-8703(87)90617-x · Cited by 23 · see citing works
HAVINGA, J. R., Lohse, P., & Beisiegel†, U. (1987) · FEBS Letters · doi:10.1016/0014-5793(87)80705-6 · Cited by 18 · see citing works
Nanjee, M. N., & Miller, N. E. (1989) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(89)90294-4 · Cited by 16 · see citing works
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Kinoshita, M., Yokote, K., Arai, H., Iida, M., Ishigaki, Y., Ishibashi, S., Umemoto, S., Egusa, G., Ohmura, H., Okamura, T., et al. (2018) · Journal of Atherosclerosis and Thrombosis · doi:10.5551/jat.gl2017 · Cited by 813 · see citing works
- Authors
- Rudling, M. J., Reihnér, E., Einarsson, K., Ewerth, S., & Angelin, B.
- Affiliations
- Karolinska Institutet (SE)
- ORCID
- Angelin, B
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1990-05
- Volume
- 87
- Issue
- 9
- Pages
- 3469-3473
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC53922
- OpenAlex
- W1998600489
- Cited by
- 112
- References
- 36
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 3 · 2016: 3 · 2015: 4 · 2014: 3 · 2013: 4 · 2012: 2
- FWCI
- 11.31
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Keywords
- LDL receptor, hepatic LDL receptor, receptor regulation, cholestyramine, LDL catabolism, adrenal gland, liver, solid tumors
- MeSH terms
- Adrenal Cortex, Adult, Aged, Cholelithiasis, Edetic Acid, Female, Gene Expression Regulation, Humans, Kinetics, Lipoproteins, LDL, Liver, Male, Middle Aged, Neoplasms, Organ Specificity, Receptors, LDL, Reference Values, Thermodynamics
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