Cited by 72Hemoglobin structure and functionProtein Structure and Dynamicsthermodynamics and calorimetric analyses

Application of linear free energy relations to protein conformational changes: the quaternary structural change of hemoglobin

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Eaton, W A, Henry, E R, Hofrichter, J·1991·Proceedings of the National Academy of Sciences

Abstract

The transition state for the R in equilibrium with T quaternary conformational change of hemoglobin has thermodynamic properties much closer to those of the R conformation than to those of the T conformation. This finding is based on a comparison of activation and equilibrium enthalpy and entropy changes and on the observation of a linear free energy relationship between quaternary rate and equilibrium constants. A previous theoretical study [Janin, J. & Wodak, S. J. (1985) Biopolymers 24, 509-526], using a highly simplified energy function, suggests that the R-like transition state is the result of a reaction pathway with the maximum buried surface area between alpha beta dimers.

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Crossref · 10.1073/pnas.88.10.4472

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Authors
Eaton, W. A., Henry, E. R., & Hofrichter, J.
Affiliations
National Institutes of Health (US)
Venue
Proceedings of the National Academy of Sciences
Published
1991-05-15
Volume
88
Issue
10
Pages
4472-4475
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2000782090
Cited by
72
References
35
Citations by year
2023: 4 · 2021: 3 · 2020: 5 · 2019: 3 · 2016: 1 · 2015: 2 · 2014: 1 · 2013: 3
FWCI
1.48
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Physical Sciences › Chemistry › Physical and Theoretical Chemistry › thermodynamics and calorimetric analyses
Keywords
hemoglobin, quaternary structure, R state, T state, conformational change, transition state, linear free energy relationships, activation enthalpy, activation entropy, alpha beta dimer, buried surface area, protein conformational dynamics
MeSH terms
Allosteric Regulation, Hemoglobins, Humans, Kinetics, Protein Conformation, Thermodynamics, Macromolecular Substances

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