Avian osteoclasts as estrogen target cells.
Open accessAbstract
Although in vivo effects of 17 beta-estradiol (estrogen) on bone turnover have been shown to occur mainly through influences on osteoclast-mediated bone resorption, the mechanism by which estrogen reduces bone resorption is unclear. To approach this question, we have examined authentic osteoclasts for evidence of a direct osteoclast response to estrogen in vitro. Highly purified (greater than (90%) viable avian osteoclasts from birds maintained on a low calcium diet were obtained using an osteoclast-specific monoclonal antibody coupled to magnetic beads. Isolated cells were either analyzed directly for estrogen receptor (ER) levels or cultured to assess the biological effects of estrogen. Northern blot analysis revealed a 5.2-kilobase mRNA that hybridized with a cDNA to human ER mRNA in the osteoclasts. An anti-human ER antibody recognized proteins of 66 kDa and 140 kDa in osteoclast extracts by Western blot analysis. The 66-kDa size is in close agreement with the reported size of the human ER. Nuclear binding of estrogen to intact viable osteoclasts was steroid-specific and saturable, with 5662 +/- 1420 molecules bound per nucleus (mean +/- SEM). In vitro estrogen responses in osteoclasts included a dose-dependent decrease in resorption as well as an increase in nuclear protooncogene mRNA levels. These observations indicate that osteoclasts are capable of directly responding to estrogen in vivo.
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Estrogen acts directly on bone cells via estrogen receptors α and β present on these cells.
“Estrogen acts directly on bone cells by its estrogen receptors α and β present on these cells [162].”
From Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells (2015) · cites this paper as [162]
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- Authors
- Oursler, M. J., Osdoby, P., Pyfferoen, J., Riggs, B. L., & Spelsberg, T. C.
- Affiliations
- Mayo Clinic (US)
- ORCID
- Osdoby, P
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1991-08
- Volume
- 88
- Issue
- 15
- Pages
- 6613-6617
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC52137
- OpenAlex
- W2043144329
- Cited by
- 395
- References
- 25
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 5 · 2023: 2 · 2022: 6 · 2021: 6 · 2020: 6 · 2019: 10 · 2018: 4 · 2017: 5 · 2016: 6 · 2015: 6 · 2014: 4 · 2013: 7 · 2012: 10
- FWCI
- 5.02
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (K04-AM147, 5r01ar032927-12, AG 04875)
- National Institute on Aging (AG 04875, P01AG004875)
- National Cancer Institute
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR32927)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- osteoclasts, estrogen, estrogen receptor, bone resorption, bone turnover, estrogen receptor mRNA, nuclear binding, low calcium diet
- MeSH terms
- Animals, Binding, Competitive, Birds, Bone Resorption, Calcium, Cell Nucleus, Cells, Cultured, DNA-Binding Proteins, Estradiol, Humans, Kinetics, Osteoclasts, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogenes, Receptors, Estrogen, RNA, Messenger, Transcription Factors, Blotting, Northern, Proto-Oncogene Proteins c-jun, Proto-Oncogene Proteins c-fos
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