Pyridine nucleotide redox state parallels production of aldosterone in potassium-stimulated adrenal glomerulosa cells.
Open accessAbstract
Extracellular potassium ions (K+) raise the intracellular concentration of free Ca2+ ([Ca2+]i) by gating voltage-dependent Ca2+ channels and stimulate aldosterone production in adrenal glomerulosa cells. The pathway leading from calcium influx to increased steroid synthesis has not been completely elucidated. In the present study we demonstrate that the reduction of pyridine nucleotides known to be required for steroid hydroxylation is enhanced by K+ (4.1-8.4 mM) in single rat glomerulosa cells. The action of K+ was strictly dependent on the presence of extracellular Ca2+. Amytal, a blocker of site I of the mitochondrial respiratory chain, abolished the K+ effect, indicating a mitochondrial origin for the recorded changes. Supraphysiological K+ concentration (18 mM) resulted in a further increase in [Ca2+]i, while steroidogenesis was decreased as measured in cell suspensions. However, a possible explanation for this dichotomy is provided by the finding that the level of reduced pyridine nucleotides also decreased at supraphysiological K+ concentration.
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- Authors
- Pralong, W. F., Hunyady, L., Várnai, P., Wollheim, C. B., & Spät, A.
- Affiliations
- University of Geneva (CH)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1992-01
- Volume
- 89
- Issue
- 1
- Pages
- 132-136
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC48190
- OpenAlex
- W2020886088
- Cited by
- 104
- References
- 31
- Citations by year
- 2025: 1 · 2023: 2 · 2021: 1 · 2018: 2 · 2017: 2 · 2016: 7 · 2015: 2 · 2014: 2 · 2013: 4 · 2012: 6
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal Regulation and Hypertension
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- aldosterone biosynthesis, adrenal glomerulosa cells, potassium stimulation, intracellular calcium, steroidogenesis, mitochondrial respiratory chain, amytal, voltage-gated calcium channels, steroid hydroxylation, extracellular calcium
- MeSH terms
- Aldosterone, Amobarbital, Animals, Calcium, Cytosol, Dose-Response Relationship, Drug, Male, Mitochondria, NAD, NADP, Oxidation-Reduction, Potassium, Rats, Inbred Strains, Time Factors, Zona Glomerulosa, Rats, In Vitro Techniques
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