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Cited by 120Neuropeptides and Animal PhysiologyNeuroendocrine regulation and behaviorCardiovascular, Neuropeptides, and Oxidative Stress Research

The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.

Evolution of the vasopressin/oxytocin superfamily: characterization of a cDNA encoding a vasopressin-related precursor, preproconopressin, from the mollusc Lymnaea stagnalis.

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van Kesteren, R E, Smit, A B, Dirks, R W, de With, N D, Geraerts, W P, Joosse, J·1992·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.89.10.4593PMC PMC49129
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Abstract

Although the nonapeptide hormones vasopressin, oxytocin, and related peptides from vertebrates and some nonapeptides from invertebrates share similarities in amino acid sequence, their evolutionary relationships are not clear. To investigate this issue, we cloned a cDNA encoding a vasopressin-related peptide, Lys-conopressin, produced in the central nervous system of the gastropod mollusc Lymnaea stagnalis. The predicted preproconopressin has the overall architecture of vertebrate preprovasopressin, with a signal peptide, Lys-conopressin, that is flanked at the C terminus by an amidation signal and a pair of basic residues, followed by a neurophysin domain. The Lymnaea neurophysin and the vertebrate neurophysins share high sequence identity, which includes the conservation of all 14 cysteine residues. In addition, the Lymnaea neurophysin possesses unique structural characteristics. It contains a putative N-linked glycosylation site at a position in the vertebrate neurophysins where a strictly conserved tyrosine residue, which plays an essential role in binding of the nonapeptide hormones, is found. The C-terminal copeptin homologous extension of the Lymnaea neurophysin has low sequence identity with the vertebrate counterparts and is probably not cleaved from the prohormone, as are the mammalian copeptins. The conopressin gene is expressed in only a few neurons in both pedal ganglia of the central nervous system. The conopressin transcript is present in two sizes, due to alternative use of polyadenylylation signals. The data presented here demonstrate that the typical organization of the prohormones of the vasopressin/oxytocin superfamily must have been present in the common ancestors of vertebrates and invertebrates.

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Metadata

Authors
van Kesteren, R. E., Smit, A. B., Dirks, R. W., de With, N. D., Geraerts, W. P., & Joosse, J.
Affiliations
  • Faculty of Biology, Vrije Universiteit, Amsterdam, The Netherlands.
  • Vrije Universiteit Amsterdam (NL)
ORCID
Smit, A B
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1992-05-15
Volume
89
Issue
10
Pages
4593-4597
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.89.10.4593
PMC
PMC49129
OpenAlex
W2155375581
Cited by
120
References
27
Citations by year
2026: 1 · 2025: 2 · 2023: 2 · 2022: 3 · 2021: 5 · 2020: 6 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 6 · 2015: 6 · 2014: 2 · 2013: 2 · 2012: 2
FWCI
2.58
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Neuroendocrine regulation and behavior
  • Health Sciences › Medicine › Surgery › Cardiovascular, Neuropeptides, and Oxidative Stress Research
Keywords
Lymnaea stagnalis, neurophysin, copeptin, N-glycosylation, alternative polyadenylation, pedal ganglia
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, DNA, Biological Evolution, Multigene Family, Humans, Lymnaea, Molecular Sequence Data, Oligodeoxyribonucleotides, Oxytocin, Protein Precursors, RNA, Messenger, Sequence Homology, Nucleic Acid, Vasopressins, Vertebrates, Restriction Mapping, Polymerase Chain Reaction

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