The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function.
Open accessAbstract
We have developed an electrical method to study endothelial cell shape changes in real time in order to examine the mechanisms of alterations in the endothelial barrier function. Endothelial shape changes were quantified by using a monolayer of endothelial cells grown on a small (10(-3) cm2) evaporated gold electrode and measuring the changes in electrical impedance. Bovine pulmonary microvessel endothelial cells and bovine pulmonary artery endothelial cells were used to study the effects of alpha-thrombin on cell-shape dynamics by the impedance measurement. alpha-Thrombin produced a dose-dependent decrease in impedance that occurred within 0.5 min in both cell types, indicative of retraction of endothelial cells and widening of interendothelial junctions because of "rounding up" of the cells. The alpha-thrombin-induced decrease in impedance persisted for approximately 2 hr, after which the value recovered to basal levels. Pretreatment of endothelial cells with the protein kinase C inhibitor, calphostin C, or with 8-bromoadenosine 3',5'-cyclic monophosphate prevented the decreased impedance, suggesting that the endothelial cell change is modulated by activation of second-messenger pathways. The alpha-thrombin-induced decrease in impedance was in agreement with the previously observed increases in transendothelial albumin permeability and evidence of formation of intercellular gaps after alpha-thrombin challenge. The impedance measurement may be a valuable in vitro method for the assessment of mechanisms of decreased endothelial barrier function occurring with inflammatory mediators. Since the rapidly occurring changes in endothelial cell shape in response to mediators such as thrombin are mediated activation of second-messenger pathways, the ability to monitor endothelial cell dynamics in real time may provide insights into the signal-transduction events mediating the increased endothelial permeability.
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Cites
Vu, T.-K. H., Hung, D. T., Wheaton, V. I., & Coughlin, S. R. (1991) · Cell · doi:10.1016/0092-8674(91)90261-v · Cited by 2,970
Focal Adhesions: Transmembrane Junctions Between the Extracellular Matrix and the Cytoskeleton
Burridge, K., Fath, K., Kelly, T., Nuckolls, G., & Turner, C. (1988) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.04.110188.002415 · Cited by 2,120
Kobayashi, E., Nakano, H., Morimoto, M., & Tamaoki, T. (1989) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(89)90028-4 · Cited by 1,108
Micromotion of mammalian cells measured electrically.
Giaever, I., & Keese, C. R. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.17.7896 · Cited by 828
Self-citationMonitoring fibroblast behavior in tissue culture with an applied electric field.
Giaever, I., & Keese, C. R. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.12.3761 · Cited by 608
Self-citationInhibition of protein kinase C by calphostin C is light-dependent
Bruns, R. F., Miller, F. D., Merriman, R. L., Howbert, J. J., Heath, W. F., Kobayashi, E., Takahashi, I., Tamaoki, T., & Nakano, H. (1991) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(91)90922-t · Cited by 367
Thrombin‐induced increase in albumin permeability across the endothelium
Garcia, J. G. N., Siflinger‐Birnboim, A., Bizios, R., Del Vecchio, P. J., Fenton, J. W., & Malik, A. B. (1986) · Journal of Cellular Physiology · doi:10.1002/jcp.1041280115 · Cited by 305
Self-citationRotrosen, D., & Gallin, J. I. (1986) · The Journal of cell biology · doi:10.1083/jcb.103.6.2379 · Cited by 293
Use of Electric Fields to Monitor the Dynamical Aspect of Cell Behavior in Tissue Culture
Giaever, I., & Keese, C. R. (1986) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.1986.325896 · Cited by 275
Self-citationIncreased endothelial albumin permeability mediated by protein kinase C activation.
Lynch, J. J., Ferro, T. J., Blumenstock, F. A., Brockenauer, A. M., & Malik, A. B. (1990) · Journal of Clinical Investigation · doi:10.1172/jci114663 · Cited by 266
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Cited by
A morphological biosensor for mammalian cells
Giaever, I., & Keese, C. R. (1993) · Nature · doi:10.1038/366591a0 · Cited by 770
Self-citationRegulation of vascular endothelial barrier function
Lum, H., & Malik, A. B. (1994) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.1994.267.3.l223 · Cited by 470
Self-citationApplication of Real-Time Cell Electronic Sensing (RT-CES) Technology to Cell-Based Assays
Solly, K., Wang, X., Xu, X., Strulovici, B., & Zheng, W. (2004) · ASSAY and Drug Development Technologies · doi:10.1089/adt.2004.2.363 · Cited by 381
Good, R. B., Gilbane, A. J., Trinder, S. L., Denton, C. P., Coghlan, G., Abraham, D. J., & Holmes, A. M. (2015) · The American Journal of Pathology · doi:10.1016/j.ajpath.2015.03.019 · Cited by 349
Bioelectrical impedance assay to monitor changes in cell shape during apoptosis
Arndt, S., Seebach, J., Psathaki, K., Galla, H.-J., & Wegener, J. (2004) · Biosensors and Bioelectronics · doi:10.1016/s0956-5663(03)00269-0 · Cited by 318
Metadata
- Authors
- Tiruppathi, C., Malik, A. B., Del Vecchio, P. J., Keese, C. R., & Giaever, I.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1992-09
- Volume
- 89
- Issue
- 17
- Pages
- 7919-7923
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1973054747
- Cited by
- 433
- References
- 25
- FWCI
- 0.82
- Type
- journal article
- Language
- en
- Open access
- green
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- Tiruppathi, C., Malik, A. B., Del Vecchio, P. J., Keese, C. R., & Giaever, I. (1992). Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.89.17.7919
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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