Cited by 96Microtubule and mitosis dynamicsDevelopmental Biology and Gene RegulationGenetic and Kidney Cyst Diseases

Cloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain

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Patel, N, Thierry-Mieg, D, Mancillas, J R·1993·Proceedings of the National Academy of Sciences

Abstract

An additional genetic locus in Caenorhabditis elegans, unc-116, was identified in a screen for mutations resulting in defective locomotion. unc-116 was cloned by use of a transposon insertion mutant and the physical and genetic map of the genome. The cDNA sequence predicts an 815-amino acid protein. Based upon sequence comparison and secondary structure predictions, unc-116 encodes all three domains of the kinesin heavy chain: the motor, stalk, and tail. While the motor and tail domains have a high degree of identity to the equivalent domains of cloned kinesin heavy chains, the rodII domain of the stalk is significantly shorter than those previously reported and is not predicted to form a coiled-coil alpha-helix. Analysis of mutational defects in two C. elegans genes encoding anterograde motor molecules, unc-116 and unc-104, should provide insight into the in vivo functions of these members of the kinesin heavy chain superfamily.

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Crossref · 10.1073/pnas.90.19.9181

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Authors
Patel, N., Thierry-Mieg, D., & Mancillas, J. R.
Affiliations
  • University of California, Los Angeles (US)
  • Molecular Biology Institute, University of California, Los Angeles 90024.
Venue
Proceedings of the National Academy of Sciences
Published
1993-10
Volume
90
Issue
19
Pages
9181-9185
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2072678197
Cited by
96
References
37
Citations by year
2026: 3 · 2025: 5 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 4 · 2012: 2
FWCI
1.64
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • W. M. Keck Foundation
  • U.S. Public Health Service
  • National Institute of General Medical Sciences (GM-07185, T32GM007185)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
kinesin heavy chain, Caenorhabditis elegans, insertional mutagenesis, transposon mutants, kinesin motor domain, stalk domain, unc-104, cDNA cloning
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Cloning, Molecular, DNA, Genetic Markers, Humans, Genetic Linkage, Molecular Sequence Data, Polymorphism, Restriction Fragment Length, Blotting, Southern, Mutagenesis, Insertional, Kinesins, Caenorhabditis elegans, Sequence Homology, Amino Acid, Protein Structure, Secondary, DNA, Complementary, Macromolecular Substances

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