Cloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain
Open accessAbstract
An additional genetic locus in Caenorhabditis elegans, unc-116, was identified in a screen for mutations resulting in defective locomotion. unc-116 was cloned by use of a transposon insertion mutant and the physical and genetic map of the genome. The cDNA sequence predicts an 815-amino acid protein. Based upon sequence comparison and secondary structure predictions, unc-116 encodes all three domains of the kinesin heavy chain: the motor, stalk, and tail. While the motor and tail domains have a high degree of identity to the equivalent domains of cloned kinesin heavy chains, the rodII domain of the stalk is significantly shorter than those previously reported and is not predicted to form a coiled-coil alpha-helix. Analysis of mutational defects in two C. elegans genes encoding anterograde motor molecules, unc-116 and unc-104, should provide insight into the in vivo functions of these members of the kinesin heavy chain superfamily.
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- Authors
- Patel, N., Thierry-Mieg, D., & Mancillas, J. R.
- Affiliations
- University of California, Los Angeles (US)
- Molecular Biology Institute, University of California, Los Angeles 90024.
- ORCID
- Mancillas, J R
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1993-10
- Volume
- 90
- Issue
- 19
- Pages
- 9181-9185
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC47526
- OpenAlex
- W2072678197
- Cited by
- 96
- References
- 37
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 4 · 2012: 2
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- W. M. Keck Foundation
- U.S. Public Health Service
- National Institute of General Medical Sciences (GM-07185, T32GM007185)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Keywords
- kinesin heavy chain, Caenorhabditis elegans, insertional mutagenesis, transposon mutants, kinesin motor domain, stalk domain, unc-104, cDNA cloning
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Cloning, Molecular, DNA, Genetic Markers, Humans, Genetic Linkage, Molecular Sequence Data, Polymorphism, Restriction Fragment Length, Blotting, Southern, Mutagenesis, Insertional, Kinesins, Caenorhabditis elegans, Sequence Homology, Amino Acid, Protein Structure, Secondary, DNA, Complementary, Macromolecular Substances
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