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Krüppel-associated boxes are potent transcriptional repression domains.
Open accessAbstract
The Krüppel-associated box (KRAB) is a highly conserved, 75-aa region containing two predicted amphipathic alpha-helices. The KRAB domain is present in the amino-terminal regions of more than one-third of all Krüppel-class Cys2His2 zinc finger proteins and is conserved from yeast to man; however, its function is unknown. Here it is shown that the KRAB domain functions as a DNA binding-dependent transcriptional repressor when fused to a heterologous DNA-binding domain from the yeast GAL4 protein. A 45-aa segment containing one of the predicted KRAB amphipathic helices was necessary and sufficient for repression. Amino acid substitutions in the predicted helix abolished the repression function. These results assign a function, transcriptional repression, to the highly conserved KRAB box and define a minimal repression domain which may aid in identifying mechanisms of repression.
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Berger, S. L., Piña, B., Silverman, N., Marcus, G. A., Agapite, J., Regier, J. L., Triezenberg, S. J., & Guarente, L. (1992) · Cell · doi:10.1016/0092-8674(92)90100-q · Cited by 435
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“…have been fused to transcription activators and repressors to modulate gene expression (Cong et al., 2012; Deuschle et al., 1995; Gossen andBujard, 1992; Hathaway et al., 2012; Maeder et al., 2013; Margolin et al., 1994; PerezPinera et al., 2013; Sadowski et al., 1988; Zhang et al., 2000).”
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Metadata
- Authors
- Margolin, J. F., Friedman, J. R., Meyer, W. K., Vissing, H., Thiesen, H. J., & Rauscher, F. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-05-10
- Volume
- 91
- Issue
- 10
- Pages
- 4509-4513
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2056793372
- Cited by
- 618
- References
- 1
- FWCI
- 6.86
- Type
- journal article
- Language
- en
- Open access
- green
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- Margolin, J. F., Friedman, J. R., Meyer, W. K., Vissing, H., Thiesen, H. J., & Rauscher, F. J. (1994). Krüppel-associated boxes are potent transcriptional repression domains. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.91.10.4509
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