Cited by 189DNA Repair MechanismsGenetic factors in colorectal cancerEnzyme Structure and Function

Formation of a ternary complex by human XPA, ERCC1, and ERCC4(XPF) excision repair proteins

Open access
Park, C H, Sancar, A·1994·Proceedings of the National Academy of Sciences

Abstract

The xeroderma pigmentosum complementation group A (XP-A) protein, XPA, has recently been expressed in Escherichia coli in a soluble and fully functional form. An affinity column was prepared by linking the XPA protein to a solid support. When HeLa cell-free extract capable of excision repair was applied to the column, > 99.9% of the proteins were in the flow-through. However, the flow-through fraction lacked excision activity. The activity was restored by adding the high salt (1 M KCl) eluate of the column to the flow-through fraction. The XPA protein-bound fraction was tested for specific proteins by an in vitro complementation assay with a panel of cell-free extracts from DNA repair-deficient human and rodent cell lines. The XPA-bound fraction complemented cell-free extracts of excision repair cross-complementing 1 (ERCC-1), ERCC-4 (XP-F), and XP-A mutants. We conclude that the XPA damage recognition protein makes a ternary complex with the ERCC1/ERCC4(XPF) heterodimer with a potential nuclease function.

Integrity signals

Registry

Crossref · 10.1073/pnas.91.11.5017

Cites

26 of 26 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Park, C. H., & Sancar, A.
Affiliations
University of North Carolina at Chapel Hill (US)
Venue
Proceedings of the National Academy of Sciences
Published
1994-05-24
Volume
91
Issue
11
Pages
5017-5021
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2091370689
Cited by
189
References
26
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 2 · 2019: 3 · 2018: 6 · 2016: 4 · 2015: 1 · 2014: 4 · 2013: 4 · 2012: 6
FWCI
7.21
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Carolina Digital Repository (University of North Carolina at Chapel Hill) (submittedVersion)
  • UNC Libraries
Funders
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM032833, GM32833)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
Keywords
XPA, ERCC1, xeroderma pigmentosum, nucleotide excision repair, ternary complex, DNA damage recognition, nuclease activity, complementation assay, cell-free extracts, affinity purification, HeLa cells
MeSH terms
Animals, Cell-Free System, Chromatography, Affinity, Cloning, Molecular, DNA Damage, DNA Repair, DNA-Binding Proteins, Endonucleases, Escherichia coli, Exonucleases, Genetic Complementation Test, HeLa Cells, Humans, Protein Binding, Proteins, Xeroderma Pigmentosum Group A Protein

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review