The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Fatty acid synthesis: a potential selective target for antineoplastic therapy
Open accessAbstract
OA-519 is a prognostic molecule found in tumor cells from breast cancer patients with markedly worsened prognosis. We purified OA-519 from human breast carcinoma cells, obtained its peptide sequence, and unambiguously identified it as fatty acid synthase through sequence homology and enzymology. Tumor fatty acid synthase is an approximately 270-kDa polypeptide which specifically abolished immunostaining of human breast cancers by anti-OA-519 antibodies. Tumor fatty acid synthase oxidized NADPH in a malonyl-CoA-dependent fashion and synthesized fatty acids composed of 80% palmitate, 10% myristate, and 10% stearate from acetyl-CoA, malonyl-CoA, and NADPH with a specific activity of 624 nmol of NADPH oxidized per min per mg. Tumor cell lines with elevated fatty acid synthase showed commensurate increases in incorporation of [U-14C]acetate into acylglycerols demonstrating that fatty acid synthase increases occur in the context of overall increases in endogenous fatty acid synthesis. Cerulenin inhibited acylglycerol synthesis in tumor cells and fibroblast controls in a dose-dependent fashion and also caused a growth inhibition which generally paralleled the level of endogenous fatty acid synthesis. Supraphysiologic levels of palmitate, 14 microM in dimethyl sulfoxide, significantly reversed the growth inhibition caused by cerulenin at concentrations of up to 5 micrograms/ml, indicating that cerulenin-mediated growth inhibition was due to fatty acid synthase inhibition.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The text we found for this paper looks like another document, so the self-check wasn’t run.
Citations
Cites
Cleveland, D. W., Fischer, S. G., Kirschner, M. W., & Laemmli, U. K. (1977) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)75212-0 · Cited by 5,556
Fatty acid synthase, a proficient multifunctional enzyme
Wakil, S. J. (1989) · Biochemistry · doi:10.1021/bi00437a001 · Cited by 823
A Practical Guide to Protein and Peptide Purification for Microsequencing
(1993) · Elsevier eBooks · doi:10.1016/c2009-0-03542-9 · Cited by 502
The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis
Ōmura, S. (1976) · Bacteriological Reviews · doi:10.1128/br.40.3.681-697.1976 · Cited by 403
Fletcher, J., Spector, A. A., & Ashbrook, J. D. (1971) · Biochemistry · doi:10.1021/bi00793a011 · Cited by 270
Stremmel, W., Strohmeyer, G. W., & Berk, P. D. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.11.3584 · Cited by 210
WEISS, L., HOFFMANN, G. E., Schreiber, R., Andres, H. H., Fuchs, E., KÖRBER, E., & Kolb, H. J. (1986) · Biological Chemistry Hoppe-Seyler · doi:10.1515/bchm3.1986.367.2.905 · Cited by 190
Binding Site of Cerulenin in Fatty Acid Synthetase1
Funabashi, H., Kawaguchi, A., Tomoda, H., Ōmura, S., Okuda, S., & Iwasaki, S. (1989) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a122739 · Cited by 179
Effects of free fatty acids on the growth of normal and neoplastic rat mammary epithelial cells.
Wicha, M. S., Liotta, L. A., & Kidwell, W. R. (1979) · PubMed · Cited by 169
Molecular cloning and sequencing of cDNAs encoding the entire rat fatty acid synthase.
Amy, C. M., Witkowski, A., Naggert, J. Κ., Williams, B., Randhawa, Z. I., & Smith, S. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.9.3114 · Cited by 161
Cited by
Sorted by citations
Items 701–718 of 718
Lipid metabolism in γδ T-cell activation: Implications for immunotherapy in ovarian cancer
Stirblyte, K., Ghanem, Y. K., Bates, M., Gray, S. G., Saadeh, F. A., Martin, C. M., O’Toole, S. A., O’Leary, J. J., Doherty, D. G., & Mohamed, B. M. (2026) · Translational Oncology · doi:10.1016/j.tranon.2026.102953
Diversity within ovarian cancer subtypes and their tumor microenvironment
Winstone, L., Bevelander, B. B., & Chamberlain, M. D. (2026) · Frontiers in Oncology · doi:10.3389/fonc.2026.1876327
Chen, H., Cui, X.-N., & Deng, M. (2026) · Neurochemistry International · doi:10.1016/j.neuint.2026.106255
Targeting ferroptosis in cancer: molecular mechanisms and emerging therapeutic strategies
Khaledi, M., Ahani, H., Tohid, F. R., Kazemi, F., Amirkhani, E., Daliri, Z., Yadegari, S., Moarefvand, K., Poureslamfar, B., Sameni, F., et al. (2026) · Biochemistry and Biophysics Reports · doi:10.1016/j.bbrep.2026.102825
The Lipid Line of Defence: Monounsaturated Fatty Acids (MUFAs) in Cancer and Ferroptosis
Baadkar, S. D., Kandettu, A., Ray, S., & Chakrabarty, S. (2026) · Oxidative Medicine and Cellular Longevity · doi:10.1155/omcl/9604592
The tumor macroenvironment as cancer metabolism’s next frontier
Grenell, A. B., Rowles, J. L., Fowle-Grider, R., Cao, Y., Shriver, L. P., & Patti, G. J. (2026) · Trends in cancer · doi:10.1016/j.trecan.2026.09.007
BindingDB (2014) · UC San Diego · doi:10.7270/q2fj2fgk
BindingDB (2009) · UC San Diego · doi:10.7270/q2fx7927
BindingDB Entry 50040470: The lipogenesis pathway as a cancer target.
BindingDB (2013) · UC San Diego · doi:10.7270/q21n8261
BindingDB Entry 2815: Novel antagonists of the thioesterase domain of human fatty acid synthase.
BindingDB (2008) · UC San Diego · doi:10.7270/q2f769v8
Fako, V. E. (2014) · IUScholarWorks (Indiana University) · doi:10.7912/c2/313
Caraceni (1998) · doi:10.1002/(sici)1097-0142(19980801)83:3
Self-citation
Metadata
- Authors
- Kuhajda, F. P., Jenner, K., Wood, F. D., Hennigar, R. A., Jacobs, L. B., Dick, J. D., & Pasternack, G. R.
- Affiliations
- Johns Hopkins University (US)
- ORCID
- Hennigar, R A
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1994-07-05
- Volume
- 91
- Issue
- 14
- Pages
- 6379-6383
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC44205
- OpenAlex
- W2090846942
- Cited by
- 705
- References
- 26
- Citations by year
- 2026: 13 · 2025: 13 · 2024: 12 · 2023: 32 · 2022: 33 · 2021: 30 · 2020: 19 · 2019: 29 · 2018: 18 · 2017: 31 · 2016: 39 · 2015: 28 · 2014: 38 · 2013: 33 · 2012: 36
- FWCI
- 3.94
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Keywords
- fatty acid synthase, breast cancer, cerulenin, antineoplastic therapy, fatty acid synthesis, palmitate, NADPH oxidation, malonyl-CoA, tumor growth inhibition
- MeSH terms
- Acetates, Antibodies, Antigens, Neoplasm, Blood Proteins, Breast Neoplasms, Carbon Radioisotopes, Cell Division, Cell Line, Cerulenin, Fatty Acids, Female, Haptoglobins, Humans, Immunohistochemistry, Kinetics, Malonyl Coenzyme A, Palmitic Acids, Prognosis, Tumor Cells, Cultured, Biomarkers, Tumor, Drug Design, Palmitic Acid, Fatty Acid Synthases
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
