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STK-1, the human homolog of Flk-2/Flt-3, is selectively expressed in CD34+ human bone marrow cells and is involved in the proliferation of early progenitor/stem cells.
Open accessAbstract
We cloned the cDNA for stem cell tyrosine kinase 1 (STK-1), the human homolog of murine Flk-2/Flt-3, from a CD34+ hematopoietic stem cell-enriched library and investigated its expression in subsets of normal human bone marrow. The cDNA encodes a protein of 993 aa with 85% identity and 92% similarity to Flk-2/Flt-3. STK-1 is a member of the type III receptor tyrosine kinase family that includes KIT (steel factor receptor), FMS (colony-stimulating factor 1R), and platelet-derived growth factor receptor. STK-1 expression in human blood and marrow is restricted to CD34+ cells, a population greatly enriched for stem/progenitor cells. Anti-STK-1 antiserum recognizes polypeptides of 160 and 130 kDa in several STK-1-expressing cell lines and in 3T3 cells transfected with a STK-1 expression vector. Antisense oligonucleotides directed against STK-1 sequences inhibited hematopoietic colony formation, most strongly in long-term bone marrow cultures. These data suggest that STK-1 may function as a growth factor receptor on hematopoietic stem and/or progenitor cells.
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“FLT3 is a key regulator of haematopoietic stem cell proliferation and dendritic cell differentiation47.”
From Rare variant associations with plasma protein levels in the UK Biobank (2023) · cites this paper as [47]
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Metadata
- Authors
- Small, D., Levenstein, M., Kim, E., Carow, C., Amin, S., Rockwell, P., Witte, L., Burrow, C., Ratajczak, M. Z., & Gewirtz, A. M.
- Affiliations
- Johns Hopkins University (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1994-01-18
- Volume
- 91
- Issue
- 2
- Pages
- 459-463
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC42968
- OpenAlex
- W2032235066
- Cited by
- 429
- References
- 40
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 6 · 2023: 4 · 2022: 8 · 2021: 11 · 2020: 12 · 2019: 8 · 2018: 2 · 2017: 9 · 2016: 4 · 2015: 3 · 2014: 14 · 2013: 7 · 2012: 15
- FWCI
- 16.04
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ca054384-03, 5p01ca051083-05)
- National Cancer Institute (CA-54384, CA-51083, CA36896)
- Topics
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
- Health Sciences › Medicine › Hematology › Platelet Disorders and Treatments
- Keywords
- CD34+ cells, hematopoietic stem cells, hematopoietic progenitor cells, bone marrow, cDNA cloning, antisense oligonucleotides, hematopoietic colony formation, growth factor receptors, KIT, FMS, platelet-derived growth factor receptor
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Bone Marrow, Bone Marrow Cells, Cloning, Molecular, Hematopoiesis, Hematopoietic Stem Cells, Humans, Molecular Sequence Data, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Antigens, CD, Gene Expression, Sequence Homology, Amino Acid, DNA, Complementary, Antigens, CD34, Receptor Protein-Tyrosine Kinases, Mice, fms-Like Tyrosine Kinase 3
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