The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter.
Open accessAbstract
Promoters whose temporal activity can be directly manipulated in transgenic animals provide a tool for the study of gene functions in vivo. We have evaluated a tetracycline-responsive binary system for its ability to temporally control gene expression in transgenic mice. In this system, a tetracycline-controlled trans-activator protein (tTA), composed of the repressor of the tetracycline-resistance operon (tet from Escherichia coli transposon Tn10) and the activating domain of viral protein VP16 of herpes simplex virus, induces transcription from a minimal promoter (PhCMV*-1; see below) fused to seven tet operator sequences in the absence of tetracycline but not in its presence. Transgenic mice were generated that carried either a luciferase or a beta-galactosidase reporter gene under the control of PhCMV*-1 or a transgene containing the tTA coding sequence under the control of the human cytomegalovirus immediate early gene 1 (hCMV IE1) promoter/enhancer. Whereas little luciferase or beta-galactosidase activity was observed in tissues of mice carrying only the reporter genes, the presence of tTA in double-transgenic mice induced expression of the reporter genes up to several thousand-fold. This induction was abrogated to basal levels upon administration of tetracycline. These findings can be used, for example, to design dominant gain-of-function experiments in which temporal control of transgene expression is required.
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Cites
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Transcriptional Activation by Tetracyclines in Mammalian Cells
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Metadata
- Authors
- Furth, P. A., St Onge, L., Böger, H., Gruss, P., Gossen, M., Kistner, A., Bujard, H., & Hennighausen, L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-09-27
- Volume
- 91
- Issue
- 20
- Pages
- 9302-9306
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2065519715
- Cited by
- 770
- References
- 25
- FWCI
- 26.34
- Type
- journal article
- Language
- en
- Open access
- green
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- Furth, P. A., St Onge, L., Böger, H., Gruss, P., Gossen, M., Kistner, A., Bujard, H., & Hennighausen, L. (1994). Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.91.20.9302
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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