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Cited by 237Neuroscience and Neuropharmacology ResearchReceptor Mechanisms and SignalingNicotinic Acetylcholine Receptors Study

Selective clustering of glutamate and gamma-aminobutyric acid receptors opposite terminals releasing the corresponding neurotransmitters.

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Craig, A M, Blackstone, C D, Huganir, R L, Banker, G·1994·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.91.26.12373PMC PMC45440
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Abstract

Several immunocytochemical and physiological studies have demonstrated a concentration of neurotransmitter receptors at postsynaptic sites on neurons, but an overall picture of receptor distribution has not emerged. In particular, it has not been clear whether receptor clusters are selectively localized opposite terminals that release the corresponding neurotransmitter. By using antibodies against the excitatory glutamate receptor subunit GluR1 and the inhibitory type A gamma-aminobutyric acid (GABA) receptor beta 2/3 subunits, we show that these different receptor types cluster at distinct postsynaptic sites on cultured rat hippocampal neurons. The GABAA receptor beta 2/3 subunits clustered on cell bodies and dendritic shafts opposite GABAergic terminals, whereas GluR1 clustered mainly on dendritic spines and was associated with glutamatergic synapses. Chronic blockade of evoked transmitter release did not block receptor clustering at postsynaptic sites. These results suggest that complex mechanisms involving nerve terminal-specific signals are required to allow different postsynaptic receptor types to cluster opposite only appropriate presynaptic terminals.

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  • Cultured hippocampal pyramidal neurons possess both spinous and nonspinous synapses, the latter often representing GABAergic synapses.

    “Cultured hippocampal pyramidal neurons possess both spinous and nonspinous synapses (Bartlett and Banker, 1984), the latter often representing GABAergic synapses that are present together with glutamatergic synapses along the dendrites (Craig et al., 1994).”

    From Regulation of F-Actin Stability in Dendritic Spines by Glutamate Receptors and Calcineurin (1998) · cites this paper as Craig et al., 1994

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Cited by

Sorted by citations

Authors
Craig, A. M., Blackstone, C. D., Huganir, R. L., & Banker, G.
Affiliations
University of Virginia (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1994-12-20
Volume
91
Issue
26
Pages
12373-12377
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.91.26.12373
PMC
PMC45440
OpenAlex
W2087045924
Cited by
237
References
2
Citations by year
2021: 2 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 4 · 2014: 1 · 2013: 4 · 2012: 3
FWCI
4.40
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institutes of Health
  • National Institute of Neurological Disorders and Stroke (NS 17112, R01NS017112)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
Keywords
glutamate receptors, GABA receptors, receptor clustering, postsynaptic localization, GluR1, hippocampal neurons, dendritic spines, glutamatergic synapses, GABAergic synapses, presynaptic terminals, neurotransmitter release
MeSH terms
Action Potentials, Animals, Evoked Potentials, Fluorescent Antibody Technique, gamma-Aminobutyric Acid, Glutamates, Hippocampus, Receptor Aggregation, Synaptic Membranes, Tetrodotoxin, Receptors, Glutamate, Receptors, GABA, Neurotransmitter Agents, Rats

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