Rat hippocampal neurons in dispersed cell culture.

Banker, Gary A.; Cowan, W. Maxwell

doi:10.1016/0006-8993(77)90594-7

Cited by 1,110Neurogenesis and neuroplasticity mechanismsNeuroscience and Neuropharmacology ResearchMemory and Neural Mechanisms

Rat hippocampal neurons in dispersed cell culture.

Banker, Gary A., Cowan, W. Maxwell·1977·Brain Research

DOI 10.1016/0006-8993(77)90594-7

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Abstract

An in vitro system has been developed for the study of isolated hippocampal neurons from 18- or 19-day rat fetuses. Following trypsinization the cells are plated out at low density on polylysine-treated coverslips in an enriched medium. The isolated neurons rapidly attach to the substrate and initiate process extension. Little reaggregation occurs and the number of non-neuronal cells present is minimal. Unless co-cultured with tissue explants the neurons survive for only a few days; in the presence of hippocampal explants the initial growth of the isolated cells is improved and their survival in culture is extended to about two weeks. Some of the cells in such cultures develop a characteristic branching pattern closely resembling that of maturing hippocampal pyramidal cells in vivo. There is a clear relationship between the stage of the cells' development and their growth in culture. Cells which had completed DNA synthesis about 48 h before dissociation, and which were in the process of migration to the cortical plate, survived best in our cultures. Early post-mitotic cells which were still within the ventricular zone and cells which had already reached the cortical plate grew poorly. This system should permit the study not only of process formation by these cells, but also of their capacity to form specific synapses in vitro and of the biochemical constituents of their surfaces.

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Hippocampal neurons go through five stages of differentiation.
“Hippocampal neurons go through five stages of differentiation (Banker and Cowan, 1977; Dotti et al., 1988).”
From Rassf5 and Ndr kinases act in a novel pathway regulating neuronal polarity through Par3 phosphorylation (2014) · cites this paper as Banker and Cowan, 1977
The majority of neurons in the hippocampus at this stage would have undergone their final cell division only 1 or 2 d before this time.
“The majority of neurons in the hippocampus at this stage would have undergone their final cell division only 1 or 2 d before this time (Banker and Cowan, 1977;Schlessinger et al., 1978).”
From Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture (1986) · cites this paper as Banker and Cowan, 1977
Within a day after plating, most neurons have begun to extend processes, typically one major process 50-100 μm long and several shorter minor processes.
“Within a day after plating, most neurons had begun to extend processes, as shown in Figure 4. Typically these included one major process, which at this stage was 50-100 pm in length, and several, much shorter, minor processes (see Banker and Cowan, 1977).”
From Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture (1986) · cites this paper as Banker and Cowan, 1977

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Authors: Banker, G. A., & Cowan, W. M.
Affiliations: Washington University in St. Louis (US)
ORCID: Banker, Gary A.
Venue: Brain Research
Publisher: Elsevier BV
Published: 1977-05
Volume: 126
Issue: 3
Pages: 397-425
ISSN: 0006-8993 (ISSN-L)
DOI: 10.1016/0006-8993(77)90594-7
OpenAlex: W1987700551
Cited by: 1,110
References: 28
Citations by year: 2026: 6 · 2025: 12 · 2024: 7 · 2023: 10 · 2022: 14 · 2021: 18 · 2020: 23 · 2019: 20 · 2018: 24 · 2017: 16 · 2016: 21 · 2015: 24 · 2014: 34 · 2013: 19 · 2012: 26
FWCI: 3.72
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Brain Research (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Keywords: hippocampal neurons, neuronal isolation, neuronal survival, cortical plate, ventricular zone, neuronal migration, DNA synthesis, synaptogenesis
MeSH terms: Animals, Axons, Cell Aggregation, Cell Division, Cell Movement, Cell Survival, Cells, Cultured, Hippocampus, Neurons, Rats

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Rat hippocampal neurons in dispersed cell culture.

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