Cited by 237Nicotinic Acetylcholine Receptors StudyMyasthenia Gravis and ThymomaMuscle Physiology and Disorders

Agrin released by motor neurons induces the aggregation of acetylcholine receptors at neuromuscular junctions

Reist, Noreen E., Werle, Michael J., McMahan, U.J.·1992·Neuron

Abstract

To test the hypothesis that agrin mediates motor neuron-induced aggregation of acetylcholine receptors (AChRs) in skeletal muscle fibers and to determine whether the agrin active in this process is released by motor neurons, we raised polyclonal antibodies to purified ray agrin that blocked its receptor aggregating activity. When the antibodies were applied to chick motor neuron--chick myotube cocultures, they inhibited the formation of AChR aggregates at and near neuromuscular contacts, demonstrating that agrin plays a role in the induction of the aggregates. Rat motor neurons, like chick motor neurons, induce AChR aggregates on chick myotubes. This effect was not inhibited by our antibodies, indicating that, although the antibodies inhibited the activity of chick agrin, they did not have a similar effect on rat agrin. We conclude that agrin released by rat motor neurons induced the chick myotubes to aggregate AChRs.

Integrity signals

Registry

Crossref · 10.1016/0896-6273(92)90200-w

Cites

21 of 21 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Neuronal-specific Agrn isoforms containing the Z exons (32 and 33) are the most potent in promoting AChR aggregation.

    “Neuronal-specific Agrn isoforms containing the Z exons (32 and 33) are the most potent in promoting AChR aggregation74–77.”

    From The neurogenetics of alternative splicing (2016) · cites this paper as [75]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Reist, N. E., Werle, M. J., & McMahan, U. J.
Affiliations
Stanford Medicine (US)
Venue
Neuron
Publisher
Elsevier BV
Published
1992-05
Volume
8
Issue
5
Pages
865-868
ISSN
0896-6273 (ISSN-L)
OpenAlex
W2068836997
Cited by
237
References
21
Citations by year
2026: 3 · 2025: 5 · 2024: 3 · 2023: 2 · 2022: 6 · 2020: 7 · 2019: 5 · 2018: 2 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 3 · 2013: 7 · 2012: 5
FWCI
8.60
Type
journal article
Language
en
Open access
closed
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
  • Health Sciences › Medicine › Neurology › Myasthenia Gravis and Thymoma
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
agrin, acetylcholine receptor aggregation, neuromuscular junction, motor neurons, polyclonal antibodies, skeletal muscle
MeSH terms
Animals, Antibodies, Cells, Cultured, Chickens, Motor Neurons, Nerve Tissue Proteins, Neuromuscular Junction, Receptors, Cholinergic, Agrin, Macromolecular Substances, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review